Sprayable hydrogel with optical mRNA nanosensors for Real-Time monitoring and healing of diabetic wounds

被引：8

作者：

Jeong, Daun ^{[1
,4
]}

Jang, Se Youn ^{[2
]}

Roh, Soonjong ^{[2
,8
]}

Choi, Ji Hye ^{[1
,4
]}

Seo, I. Ji ^{[3
]}

Lee, Jin Hyuck ^{[4
]}

Kim, Jihoon ^{[3
]}

Kwon, Ilkeun ^{[5
]}

Jung, Youngmee ^{[2
,6
]}

Hwang, Jangsun ^{[1
,4
]}

Jang, Woo Young ^{[1
,4
]}

Yoo, Jin ^{[2
,7
]}

机构：

[1] Korea Univ, Dept Orthoped Surg, Coll Med, Seoul 02841, South Korea

[2] Korea Inst Sci & Technol KIST, Biomed Res Inst, Biomat Res Ctr, Seoul 02792, South Korea

[3] Korea Inst Sci & Technol KIST, Biomed Res Inst, Adv Biomol Recognit Ctr, Seoul 02792, South Korea

[4] Korea Univ, Inst Nanobiomarker Based Med, Seoul 02841, South Korea

[5] Kyung Hee Univ, Sch Dent, Dept Dent Mat, Seoul 02447, South Korea

[6] Yonsei Univ, YU KIST Inst, Sch Elect Elect Engn, Seoul 03722, South Korea

[7] Korea Univ Sci & Technol UST, KIST Sch, Div Biomed Sci & Technol, Seoul 02792, South Korea

[8] Seoul Natl Univ, Grad Sch Convergence Sci & Technol, Dept Appl Bioengn, Seoul 08826, South Korea

来源：

CHEMICAL ENGINEERING JOURNAL | 2024年 / 493卷

基金：

新加坡国家研究基金会;

关键词：

Sprayable hydrogels; Nanosensor; Wound healing; Diabetic wound; Antimicrobial peptides; DRESSINGS; LAPONITE; INFLAMMATION; ADHESION; PECAM-1; CLAY;

D O I：

10.1016/j.cej.2024.152711

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Sprayable hydrogels offer a promising approach for treating diabetic wounds. They possess the capability to conform to irregular surfaces, create a moist environment that supports the growth of new tissue, and offer protection against infections. Yet, applying previously reported sprayable hydrogels has proven to be challenging, often necessitating the use of specialized equipment or the simultaneous injection of multiple components. In this study, we develop a user-friendly therapeutic hydrogel that can be applied using a general spray bottle. This hydrogel is formulated using the bioactive agent, LL37, which is known for its antimicrobial properties and ability to enhance angiogenesis. Additionally, optical mRNA nanosensors, NanoFlares, were incorporated into the hydrogel to measure the expression of proliferation and inflammation biomarkers, providing precise molecular-based parameters for evaluating diabetic wound status. The efficacy of LL37- and NFincorporated hydrogels in promoting in vivo wound healing in normal and diabetic mice is evaluated, and a Wound Healing Index (WHI) is presented to assist medical professionals in making informed decisions about wound care and treatment. Overall, our study demonstrates the potential of sprayable hydrogels for promoting diabetic wound healing and monitoring the diabetic wound status, which could have significant potential for future clinical applications.

引用

页数：14

共 72 条

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