Comprehensive analyses show the enhancement effect of exogenous melatonin on fluroxypyr-meptyl multiple phase metabolisms in Oryza sativa for reducing environmental risks

被引:3
作者
Chen, Zhao Jie [1 ]
Li, Si Ying [1 ]
Qu, Ya Nan [1 ]
Ai, Gan [2 ]
Wang, Yan Hui [3 ]
Pan, Dong Jin [3 ]
Wang, Hao Wen [1 ]
Lu, Dan [1 ]
Liu, Xiao Liang [1 ]
机构
[1] Guangxi Univ, Coll Agr, Guangxi Key Lab Agr Environm & Agr Prod Safety, Nanning 530004, Guangxi, Peoples R China
[2] Nanjing Agr Univ, Coll Plant Protect, Key Lab Plant Immun, Nanjing 210095, Peoples R China
[3] Guangxi Acad Agr Sci, Plant Protect Res Inst, Guangxi Key Lab Biol Crop Dis & Insect Pests, Nanning 530007, Peoples R China
基金
中国博士后科学基金;
关键词
Melatonin; Fluroxypyr-meptyl; Rice; Transcriptomic analysis; Multiple metabolism; RESIDUES; PLANTS;
D O I
10.1016/j.pestbp.2024.106021
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The role of melatonin (MT), an essential phytohormone controlling the physiological and biochemical reactions of plants to biotic and abiotic stress, in alleviating pesticide phytotoxicity remains unclear. This study explores the effects of MT (0 and 200 mg/L) and six doses of fluroxypyr-meptyl (FLUME) (0-0.14 mg/L) on the physiological response of rice (Oryza sativa). FLUME exposure inhibited the growth of rice seedlings, with MT treatment ameliorating this effect. To determine the biochemical processes and catalytic events involved in FLUME breakdown in rice, six rice root and shoot libraries exposed to either FLUME or FLUME-MT were generated and then subjected to RNA-Seq-LC-Q-TOF-HRMS/MS analyses. The results showed that 1510 root genes and 139 shoot genes exhibited higher upregulation in plants treated with an ecologically realistic FLUME concentration and MT than in those treated with FLUME alone. Gene enrichment analysis revealed numerous FLUME-degradative enzymes operating in xenobiotic tolerance to environmental stress and molecular metabolism. Regarding the FLUME degradation process, certain differentially expressed genes were responsible for producing important enzymes, such as cytochrome P450, glycosyltransferases, and acetyltransferases. Four metabolites and ten conjugates in the pathways involving hydrolysis, malonylation, reduction, glycosylation, or acetylation were characterized using LC-Q-TOF-HRMS/MS to support FLUME-degradative metabolism. Overall, external application of MT can increase rice tolerance to FLUME-induced oxidative stress by reducing phytotoxicity and FLUME accumulation. This study provides insights into MT's role in facilitating FLUME degradation, with potential implications for engineering genotypes supporting FLUME degradation in paddy crops.
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页数:11
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