Genome-wide identification of the TIFY gene family in tobacco and expression analysis in response to Ralstonia solanacearum infection

被引:6
作者
Zhang, Huifen [1 ,2 ]
Liu, Zhengwen [1 ]
Geng, Ruimei [1 ]
Ren, Min [1 ]
Cheng, Lirui [1 ]
Liu, Dan [1 ]
Jiang, Caihong [1 ]
Wen, Liuying [1 ]
Xiao, Zhiliang [1 ]
Yang, Aiguo [1 ]
机构
[1] Chinese Acad Agr Sci, Tobacco Res Inst, Key Lab Tobacco Gene Resources, Qingdao 266101, Peoples R China
[2] Grad Sch Chinese Acad Agr Sci, Grad Sch, Beijing 100081, Peoples R China
关键词
TIFY gene family; Genome-wide identification; Bacterial wilt; Tobacco; VIGS; BACTERIAL WILT; LEAF GROWTH; ARABIDOPSIS; RESISTANCE; SEQUENCE; PROTEIN; PHASE; ZIM;
D O I
10.1016/j.ygeno.2024.110823
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The TIFY gene family plays an essential role in plant development and abiotic and biotic stress responses. In this study, genome-wide identification of TIFY members in tobacco and their expression pattern analysis in response to Ralstonia solanacearum infection were performed. A total of 33 TIFY genes were identified, including the TIFY, PPD, ZIM&ZML and JAZ subfamilies. Promoter analysis results indicated that a quantity of light-response, drought-response, SA-response and JA-response cis-elements exist in promoter regions. The TIFY gene family exhibited expansion and possessed gene redundancy resulting from tobacco ploidy change. In addition, most NtTIFYs equivalently expressed in roots, stems and leaves, while NtTIFY1, NtTIFY4, NtTIFY18 and NtTIFY30 preferentially expressed in roots. The JAZ III clade showed significant expression changes after inoculation with R. solanacearum, and the expression of NtTIFY7 in resistant varieties, compared with susceptible varieties, was more stably induced. Furthermore, NtTIFY7-silenced plants, compared with the control plants, were more susceptible to bacterial wilt. These results lay a foundation for exploring the evolutionary history of TIFY gene family and revealing gene function of NtTIFYs in tobacco bacterial wilt resistance.
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页数:13
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