Rapid screening of high-protein Auxenochlorella pyrenoidosa mutant by an integrated system of atmospheric and room temperature plasma mutagenesis and high-throughput microbial microdroplet culture

被引：6

作者：

Liu, Yu ^{[1
]}

Chen, Xiao ^{[1
]}

Wei, Dong ^{[1
]}

Xing, Xinhui ^{[2
,3
]}

机构：

[1] South China Univ Technol, Guangdong Prov Key Lab Green Proc Nat Prod & Prod, Sch Food Sci & Engn, Engn Res Ctr Starch & Vegetable Prot Proc,Minist E, Wushan Rd 381, Guangzhou 510641, Peoples R China

[2] Tsinghua Univ, Inst Biochem Engn, Dept Chem Engn, Key Lab Ind Biocatalysis,Minist Educ, Beijing 100084, Peoples R China

[3] Tsinghua Shenzhen Int Grad Sch, Inst Biopharmaceut & Hlth Engn, Shenzhen 518055, Peoples R China

来源：

ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS | 2024年 / 80卷

关键词：

Auxenochlorella pyrenoidosa; High-protein mutant; Atmospheric and room temperature plasma; mutagenesis; Microbial microdroplet culture; High-throughput screening; MICROALGAE CHLORELLA-PYRENOIDOSA; AMINO-ACIDS; CULTIVATION; BIODIESEL; BIOMASS; INGREDIENT; QUALITY; GROWTH; SP;

D O I：

10.1016/j.algal.2024.103509

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Microalgae cultivated heterotrophically often exhibit high biomass concentration but low protein content. An integrated system combining atmospheric and room temperature plasma (ARTP) mutagenesis with highthroughput microbial microdroplet culture (MMC) was introduced to rapidly breed high-protein strains of Auxenochlorella pyrenoidosa for heterotrophic fermentation. Utilizing the previously identified A4-1 mutant strain as original strain, ARTP-induced mutants were screened through MMC, prioritizing mutants with rapidgrowth and high-protein via subsequent well-plate and shake flask cultivation based on evaluation of growth and biochemical composition. The MMC-8 mutant exhibited the highest protein content (63.26 % dry weight, DW) and the lowest starch content (8.59 % DW), representing a 40.11 % protein increase and a 56.24 % starch decrease relative to A4-1. MMC-8 also demonstrated superior protein quality, with a higher amino acid content (44.35 % DW) and score (95) than the A4-1. These attributes establish MMC-8 as a promising candidate for alternative protein production through heterotrophic fermentation, while the ARTP-MMC system was proved to be invaluable for the selective screening of microalgae.

引用

页数：8