Identification of key genes associated with acute myocardial infarction using WGCNA and two-sample mendelian randomization study

被引:0
|
作者
Yang, Xiaohe [1 ]
Huang, Yingtao [2 ]
Tang, Dadong [3 ]
Yue, Liangming [1 ]
机构
[1] Guangyuan Hosp Tradit Chinese Med, Dept Cardiol, Guangyuan, Peoples R China
[2] Liaoning Univ Tradit Chinese Med, Dept Orthoped, Shenyang, Peoples R China
[3] Chengdu Univ Tradit Chinese Med, Sch Clin Coll Med, Chengdu, Peoples R China
来源
PLOS ONE | 2024年 / 19卷 / 07期
关键词
TUMOR-NECROSIS-FACTOR; TNF-ALPHA; ISCHEMIA; RECOVERY; CELLS;
D O I
10.1371/journal.pone.0305532
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Objective Acute myocardial infarction (AMI) is a severe condition with high morbidity and mortality rates. This study aimed to identify hub genes potentially associated with AMI and assess their clinical utility in predicting AMI occurrence.Methods Gene microarray data were obtained from the Gene Expression Omnibus (GEO) database. Differential expression analysis and weighted gene co-expression network analysis (WGCNA) were conducted on samples from patients with AMI and control samples to identify modules significantly associated with AMI. GO and KEGG analyses were applied to investigate the potential functions of these hub genes. Lastly, the mendelian randomization (MR) method was applied to analyze the causal relationship between the hub gene TNF and AMI.Results 285 differentially expressed genes (DEGs) were identified through WCGNA and were clustered into 6 modules. The yellow module appeared most relevant to AMI. Further exploration through GO and KEGG pathway enrichment showed that key hub genes in the yellow module were linked to positive regulation of cytokine production, cytokine receptor binding, NF-kappa B signaling pathway, IL-17 signaling pathway, and TNF signaling pathway. The top 10 genes identified through Cytoscape software analysis were IL1B, TNF, TLR4, TLR2, FCGR3B, MMP9, CXCL8, TLR8, ICAM1, and JUK. Utilizing inverse variance weighting (IVW) analysis, we discovered a significant association between TNF and AMI risk, with an OR of 0.946 (95% CI = 0.911-0.984, p = 0.005).Conclusions The result of this study indicated that TNF, TLR2, TLR4, IL1B and FCGR3B may be potential biodiagnostic markers for AMI. TNF can inhibit inflammatory and oxidative stress responses in AMI, exerting a protective role in the heart.
引用
收藏
页数:16
相关论文
共 50 条
  • [1] Functional investigation and two-sample Mendelian randomization study of neuropathic pain hub genes obtained by WGCNA analysis
    Zeng, Jianfeng
    Lai, Cong
    Luo, Jianwei
    Li, Li
    FRONTIERS IN NEUROSCIENCE, 2023, 17
  • [2] Allopurinol is Associated with an Increased Risk of Cerebral Infarction: A Two-Sample Mendelian Randomization Study
    Ma, Xiao-Na
    Shi, Mei-Feng
    Feng, Wei
    Chen, Shu-Lin
    Zhong, Xiao-Qin
    Lin, Chang-Song
    Xu, Qiang
    ACS OMEGA, 2024, 9 (31): : 33826 - 33832
  • [3] The causal relationship between triglycerides and myocardial infarction: A two-sample Mendelian randomization
    Kong, Lu
    Yang, Zhong-Bin
    Chen, Xie-Hui
    Quan, Xiao-Qing
    Liu, Hong-Tao
    Qiu, Ai-Ping
    MEDICINE, 2024, 103 (37)
  • [4] Bidirectional two-sample Mendelian randomization study of causality between rheumatoid arthritis and myocardial infarction
    Guo, Hao-Yang
    Wang, Wei
    Peng, Hui
    Yuan, Hui
    FRONTIERS IN IMMUNOLOGY, 2022, 13
  • [5] Myocardial infarction and stroke risks in multiple sclerosis patients: A two-sample Mendelian randomization study
    Peng, Haoxin
    Wu, Xiangrong
    Wen, Yaokai
    Lin, Jinsheng
    Guan, Wenhui
    MULTIPLE SCLEROSIS AND RELATED DISORDERS, 2022, 58
  • [6] Causal relationship between breakfast skipping and myocardial infarction: Two-sample Mendelian randomization
    Guo, Yuli
    Lv, Luo
    Gao, Hui
    Feng, Ruiting
    Guo, Min
    MEDICINE, 2024, 103 (30)
  • [7] A two-sample Mendelian randomization study of atherosclerosis and dementia
    Zhang, Qiaoyun
    Wu, Guangheng
    Zhang, Xiaoyu
    Wang, Sheng
    Wang, Youxin
    ISCIENCE, 2023, 26 (11)
  • [8] Lipoprotein(a) and stroke: a two-sample Mendelian randomization study
    Huang, Yi
    Zhang, Ruijie
    Han, Liyuan
    Wu, Yiwen
    Deng, Xinpeng
    Xu, Tianqi
    Wu, Yuefei
    Gao, Xiang
    Zhou, Chenhui
    Sun, Jie
    FRONTIERS IN AGING NEUROSCIENCE, 2023, 15
  • [9] Diabetes and osteoporosis: a two-sample mendelian randomization study
    Qu, Yu-Dun
    Zhu, Zhao-Hua
    Li, Jia-Xuan
    Zhang, Wei
    Chen, Qi
    Xia, Chang-Liang
    Ma, Jun-Nan
    Ou, Shuan-Ji
    Yang, Yang
    Qi, Yong
    Xu, Chang-Peng
    BMC MUSCULOSKELETAL DISORDERS, 2024, 25 (01)
  • [10] Causal effects for genetic variants of osteoprotegerin on the risk of acute myocardial infarction and coronary heart disease: A two-sample Mendelian randomization study
    Chao, Peng
    Zhang, Xueqin
    Zhang, Lei
    Cui, Xinyue
    Wang, Shanshan
    Yang, Yining
    FRONTIERS IN CARDIOVASCULAR MEDICINE, 2023, 10