Unveiling the Genomic Landscape of Intraductal Carcinoma of the Prostate Using Spatial Gene Expression Analysis

被引：5

作者：

Watanabe, Ryuta ^{[1
,2
]}

Miura, Noriyoshi ^{[1
]}

Kurata, Mie ^{[3
,4
]}

Kitazawa, Riko ^{[5
]}

Kikugawa, Tadahiko ^{[1
]}

Saika, Takashi ^{[1
]}

机构：

[1] Ehime Univ, Grad Sch Med, Dept Urol, Toon 7910295, Japan

[2] Fred Hutchinson Canc Ctr, Human Biol Div, Seattle, WA 98109 USA

[3] Ehime Univ, Grad Sch Med, Dept Analyt Pathol, Toon 7910295, Japan

[4] Ehime Univ, Proteo Sci Ctr, Div Pathol, Toon 7910295, Japan

[5] Ehime Univ Hosp, Div Diagnost Pathol, Toon 7910295, Japan

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2024年 / 25卷 / 09期

关键词：

spatial gene expression analysis; intraductal carcinoma of the prostate (IDCP); hypoxic markers; immune cells; tumor microenvironment; INTRAEPITHELIAL NEOPLASIA; INVASIVE ADENOCARCINOMA; HISTOLOGIC FEATURES; NEEDLE-BIOPSY; CANCER; PATHOLOGY; TUMORS; PTEN;

D O I：

10.3390/ijms25094818

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Intraductal carcinoma of the prostate (IDCP) has recently attracted increasing interest owing to its unfavorable prognoses. To effectively identify the IDCP-specific gene expression profile, we took a novel approach of characterizing a typical IDCP case using spatial gene expression analysis. A formalin-fixed, paraffin-embedded sample was subjected to Visium CytAssist Spatial Gene Expression analysis. IDCP within invasive prostate cancer sites was recognized as a distinct cluster separate from other invasive cancer clusters. Highly expressed genes defining the IDCP cluster, such as MUC6, MYO16, NPY, and KLK12, reflected the aggressive nature of high-grade prostate cancer. IDCP sites also showed increased hypoxia markers HIF1A, BNIP3L, PDK1, and POGLUT1; decreased fibroblast markers COL1A2, DCN, and LUM; and decreased immune cell markers CCR5 and FCGR3A. Overall, these findings indicate that the hypoxic tumor microenvironment and reduced recruitment of fibroblasts and immune cells, which reflect morphological features of IDCP, may influence the aggressiveness of high-grade prostate cancer.

引用

页数：14

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