The effect of injectable plateletrich fibrin and platelet-rich fibrin in regenerative endodontics: a comparative in vitro study

被引：1
作者：
Pan, Jing [1 ,2 ]
Luo, Linjuan [1 ,2 ]
Jiang, Zhen [1 ,2 ]
Huang, Haiyan [1 ,2 ]
Jiang, Beizhan [1 ,2 ,3 ,4 ]
机构：
[1] Tongji Univ, Stomatol Hosp, Shanghai Engn Res Ctr Tooth Restorat & Regenerat, Shanghai, Peoples R China
[2] Dent Sch Tongji Univ, Dept Pediat Dent, Shanghai, Peoples R China
[3] Tongji Univ, Stomatol Hosp, Shanghai, Peoples R China
[4] Tongji Univ, Dent Sch, Shanghai Engn Res Ctr Tooth Restorat & Regenerat, Dept Pediat Dent, Shanghai, Peoples R China
来源：
JOURNAL OF APPLIED ORAL SCIENCE | 2024年 / 32卷
关键词：
Platelet-rich fibrin; Angiogenesis; Mineralization; Regenerative endodontics; STEM-CELLS; DIFFERENTIATION;
D O I：
10.1590/1678-7757-2023-0449
中图分类号：
R78 [口腔科学];
学科分类号：
1003 ;
摘要：
Objective: To explore the feasibility of injectable platelet-rich fibrin (i-PRF) in regenerative endodontics by comparing the effect of i-PRF and platelet-rich fibrin (PRF) on the biological behavior and angiogenesis of human stem cells from the apical papilla (SCAPs). Methodology: i-PRF and PRF were obtained from venous blood by two different centrifugation methods, followed by hematoxylin-eosin (HE) staining and scanning electron microscopy (SEM). Enzyme-linked immunosorbent assay (ELISA) was conducted to quantify the growth factors. SCAPs were cultured with different concentrations of i-PRF extract (i-PRFe) and PRF extract (PRFe), and the optimal concentrations were selected using the Cell Counting Kit-8 (CCK-8) assay. The cell proliferation and migration potentials of SCAPs were then observed using the CCK-8 and Transwell assays. Mineralization ability was detected by alizarin red staining (ARS), and angiogenesis ability was detected by tube formation assay. Real-time quantitative polymerase chain reaction (RT-qPCR) was performed to evaluate the expression of genes related to mineralization and angiogenesis. The data were subjected to statistical analysis. Results: i-PRF and PRF showed a similar three-dimensional fibrin structure, while i-PRF released a higher concentration of growth factors than PRF (P<.05). 1/4x i-PRFe and 1/4x PRFe were selected as the optimal concentrations. The cell proliferation rate of the i-PRFe group was higher than that of the PRFe group (P<.05), while no statistical difference was observed between them in terms of cell mitigation (P>.05). More importantly, our results showed that i-PRFe had a stronger effect on SCAPs than PRFe in facilitating mineralization and angiogenesis, with the consistent result of RT-qPCR (P<.05). Conclusion: This study revealed that i-PRF released a higher concentration of growth factors and was superior to PRF in promoting proliferation, mineralization and angiogenesis of SCAPs, which indicates that i-PRF could be a promising biological scaffold for application in pulp regeneration.
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页数：11
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