Lycopene inhibits pyroptosis of endothelial progenitor cells induced by ox-LDL through the AMPK/mTOR/NLRP3 pathway

被引：3

作者：

Tan, Chujun ^{[2
]}

Chen, Junqiu ^{[4
]}

Tu, Tengcan ^{[2
]}

Chen, Lifang ^{[1
]}

Zou, Jun ^{[2
,3
]}

机构：

[1] Shenzhen Qianhai Shekou Free Trade Zone Hosp, Dept Cardiol, Shenzhen 528200, Peoples R China

[2] Southern Med Univ, Sch Clin Med 2, Guangzhou 510280, Peoples R China

[3] South China Univ Technol, Affiliated Hosp 6, Sch Med, Dept Cardiol, Foshan 528200, Peoples R China

[4] Chinese Acad Med Sci, Fuwai Hosp, Shenzhen Sun Yat Sen Cardiovasc Hosp, Dept Cardiol, Shenzhen 518057, Peoples R China

来源：

OPEN MEDICINE | 2024年 / 19卷 / 01期

关键词：

lycopene; AMPK/mTOR; NLRP3; ox-LDL; EPCs; NLRP3; INFLAMMASOME; OXIDATIVE STRESS; PHOSPHOCREATINE PROTECTS; ACTIVATION; INJURY; EPCS; ATHEROSCLEROSIS; OXYGENASE-1; APOPTOSIS; TOMATO;

D O I：

10.1515/med-2024-0973

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

The malfunction of endothelial progenitor cells (EPCs) due to ox-LDL is a risk contributor for arteriosclerotic disease. Meanwhile, lycopene possesses anti-inflammatory and antioxidative qualities. This investigation aimed to determine if lycopene can protect EPCs from ox-LDL-induced damage and to elucidate the underlying mechanism. The effects of lycopene on the survival, migration, and tube-forming capacity of EPCs were determined via in vitro assays. Expression of proteins related to pyroptosis and cellular proteins related to AMPK/mTOR/NLRP3 signaling was determined by western blot/flow cytometry. Our results demonstrated that lycopene treatment significantly enhanced proliferation, tube formation, and migration of EPCs stimulated by ox-LDL. Additionally, lycopene was found to suppress pyroptosis in ox-LDL-induced EPCs through the activation of AMPK, which led to the inhibition of mTOR phosphorylation and subsequent downregulation of the downstream NLRP3 inflammasome. In summary, our study suggests that lycopene mitigates ox-LDL-induced dysfunction in EPCs and inhibits pyroptosis via AMPK/mTOR/NLRP3 signaling. Our study suggests that lycopene may act as promising therapies for preventing atherosclerosis.

引用

页数：14

共 47 条

[1] Phosphocreatine protects endothelial cells from oxidized low-density lipoprotein-induced apoptosis by modulating the PI3K/Akt/eNOS pathway [J].

Ahsan, Anil ;

Han, Guozhu ;

Pan, Junfang ;

Liu, Shumin ;

Padhiar, Arshad Ahmed ;

Chu, Peng ;

Sun, Zhengwu ;

Zhang, Zonghui ;

Sun, Bin ;

Wu, Jingjun ;

Irshad, Aisha ;

Lin, Yuan ;

Peng, Jinyong ;

Tang, Zeyao .

APOPTOSIS, 2015, 20 (12) :1563-1576

[2] Endothelial progenitor cells (EPCs) in ageing and age-related diseases: How currently available treatment modalities affect EPC biology, atherosclerosis, and cardiovascular outcomes [J].

Altabas, Velimir ;

Altabas, Karmela ;

Kirigin, Lora .

MECHANISMS OF AGEING AND DEVELOPMENT, 2016, 159 :49-62

[3]

Arnett DK, 2019, CIRCULATION, V140, pE563, DOI [10.1161/CIR.0000000000000677, 10.1161/CIR.0000000000000678, 10.1016/j.jacc.2019.03.009, 10.1016/j.jacc.2019.03.010]

[4] Lycopene in association with α-tocopherol or tomato lipophilic extracts enhances acyl-platelet-activating factor biosynthesis in endothelial cells during oxidative stress [J].

Balestrieri, ML ;

De Prisco, R ;

Nicolaus, B ;

Pari, P ;

Moriello, VS ;

Strazzullo, G ;

Iorio, EL ;

Servillo, L ;

Balestrieri, C .

FREE RADICAL BIOLOGY AND MEDICINE, 2004, 36 (08) :1058-1067

[5] Tomatoes versus lycopene in oxidative stress and carcinogenesis: conclusions from clinical trials [J].

Basu, A. ;

Imrhan, V. .

EUROPEAN JOURNAL OF CLINICAL NUTRITION, 2007, 61 (03) :295-303

[6] Identification and targeting of novel CDK9 complexes in acute myeloid leukemia [J].

Beauchamp, Elspeth M. ;

Abedin, Sameem M. ;

Radecki, Sara G. ;

Fischietti, Mariafausta ;

Arslan, Ahmet Dirim ;

Blyth, Gavin T. ;

Yang, Angela ;

Lantz, Connor ;

Nelson, Alissa ;

Goo, Young Ah ;

Akpan, Imo ;

Eklund, Elizabeth A. ;

Frankfurt, Olga ;

Fish, Eleanor N. ;

Thomas, Paul M. ;

Altman, Jessica K. ;

Platanias, Leonidas C. .

BLOOD, 2019, 133 (11) :1171-1185

[7] Artemisinin Attenuated Atherosclerosis in High-Fat Diet-Fed ApoE-/- Mice by Promoting Macrophage Autophagy Through the AMPK/mTOR/ULK1 Pathway [J].

Cao, Qian ;

Du, Hongjiao ;

Fu, Xi ;

Duan, Na ;

Liu, Changhe ;

Li, Xiaodong .

JOURNAL OF CARDIOVASCULAR PHARMACOLOGY, 2020, 75 (04) :321-332

[8] Mitochondrial mutations and mitoepigenetics: Focus on regulation of oxidative stress-induced responses in breast cancers [J].

Chen, Kuo ;

Lu, Pengwei ;

Beeraka, Narasimha M. ;

Sukocheva, Olga A. ;

Madhunapantula, SubbaRao, V ;

Liu, Junqi ;

Sinelnikov, Mikhail Y. ;

Nikolenko, Vladimir N. ;

Bulygin, Kirill, V ;

Mikhaleva, Liudmila M. ;

Reshetov, Igor, V ;

Gu, Yuanting ;

Zhang, Jin ;

Cao, Yu ;

Somasundaram, Siva G. ;

Kirkland, Cecil E. ;

Fan, Ruitai ;

Aliev, Gjumrakch .

SEMINARS IN CANCER BIOLOGY, 2022, 83 :556-569

[9] Tomato and lycopene supplementation and cardiovascular risk factors: A systematic review and meta-analysis [J].

Cheng, Ho Ming ;

Koutsidis, Georgios ;

Lodge, John K. ;

Ashor, Ammar ;

Siervo, Mario ;

Lara, Jose .

ATHEROSCLEROSIS, 2017, 257 :100-108

[10] Phosphocreatine protects endothelial cells from Methylglyoxal induced oxidative stress and apoptosis via the regulation of PI3K/Akt/eNOS and NF-κB pathway [J].