Metabolome and Transcriptome Unveil the Correlated Metabolites and Transcripts with 2-acetyl-1-pyrroline in Fragrant Rice

被引:2
作者
Zeng, Yu [1 ]
Nong, Baoxuan [1 ]
Xia, Xiuzhong [1 ]
Zhang, Zongqiong [1 ]
Wang, Yuhao [1 ]
Xu, Yong [1 ]
Feng, Rui [1 ]
Guo, Hui [1 ]
Liang, Yuntao [1 ]
Chen, Can [1 ]
Liang, Shuhui [1 ]
Jiang, Xianbin [1 ]
Yang, Xinghai [1 ]
Li, Danting [1 ]
机构
[1] Guangxi Acad Agr Sci, Rice Res Inst, Key Lab Rice Genet & Breeding, Nanning 530007, Peoples R China
基金
中国国家自然科学基金;
关键词
fragrant rice; metabolome; transcriptome; WGCNA analysis; 2AP; ORYZA-SATIVA; GENE; BIOSYNTHESIS; STRINGTIE; PROLINE; MARKERS; AROMA; HISAT;
D O I
10.3390/ijms25158207
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Fragrance is a valuable trait in rice varieties, with its aroma significantly influencing consumer preference. In this study, we conducted comprehensive metabolome and transcriptome analyses to elucidate the genetic and biochemical basis of fragrance in the Shangsixiangnuo (SSXN) variety, a fragrant indica rice cultivated in Guangxi, China. Through sensory evaluation and genetic analysis, we confirmed SSXN as strongly fragrant, with an 806 bp deletion in the BADH2 gene associated with fragrance production. In the metabolome analysis, a total of 238, 233, 105 and 60 metabolic compounds exhibited significant changes at the seedling (S), reproductive (R), filling (F), and maturation (M) stages, respectively. We identified four compounds that exhibited significant changes in SSXN across all four development stages. Our analyses revealed a significant upregulation of 2-acetyl-1-pyrroline (2AP), the well-studied aromatic compound, in SSXN compared to the non-fragrant variety. Additionally, correlation analysis identified several metabolites strongly associated with 2AP, including ethanone, 1-(1H-pyrrol-2-yl)-, 1H-pyrrole, and pyrrole. Furthermore, Weighted Gene Co-expression Network Analysis (WGCNA) analysis highlighted the magenta and yellow modules as particularly enriched in aroma-related metabolites, providing insights into the complex aromatic compounds underlying the fragrance of rice. In the transcriptome analysis, a total of 5582, 5506, 4965, and 4599 differential expressed genes (DEGs) were identified across the four developmental stages, with a notable enrichment of the common pathway amino sugar and nucleotide sugar metabolism in all stages. In our correlation analysis between metabolome and transcriptome data, the top three connected metabolites, phenol-, 3-amino-, and 2AP, along with ethanone, 1-(1H-pyrrol-2-yl)-, exhibited strong associations with transcripts, highlighting their potential roles in fragrance biosynthesis. Additionally, the downregulated expression of the P4H4 gene, encoding a procollagen-proline dioxygenase that specifically targets proline, in SSXN suggests its involvement in proline metabolism and potentially in aroma formation pathways. Overall, our study provides comprehensive insights into the genetic and biochemical mechanisms underlying fragrance production in rice, laying the foundation for further research aimed at enhancing fragrance quality in rice breeding programs.
引用
收藏
页数:18
相关论文
共 56 条
[21]   2-Acetyl-1-Pyrroline Augmentation in Scented indica Rice (Oryza sativa L.) Varieties Through Δ1-Pyrroline-5-Carboxylate Synthetase (P5CS) Gene Transformation [J].
Kaikavoosi, Kayghobad ;
Kad, Trupti D. ;
Zanan, Rahul L. ;
Nadaf, Altafhusain B. .
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2015, 177 (07) :1466-1479
[22]  
Kim D, 2015, NAT METHODS, V12, P357, DOI [10.1038/NMETH.3317, 10.1038/nmeth.3317]
[23]  
Langmead B, 2012, NAT METHODS, V9, P357, DOI [10.1038/nmeth.1923, 10.1038/NMETH.1923]
[24]   Genetic Diversity Evaluation and Conservation of Kam Fragrant Glutinous Rice (Oryza sativa L.) Germplasm in Southeast Guizhou, China [J].
Lei, Qi-Yi ;
Zhou, Jiang-Ju ;
Xiong, Yong ;
Zhang, Wen-Hua ;
Luo, Jing ;
Long, Chun-Lin .
PLANTS-BASEL, 2021, 10 (09)
[25]   Roles of dietary glycine, proline, and hydroxyproline in collagen synthesis and animal growth [J].
Li, Peng ;
Wu, Guoyao .
AMINO ACIDS, 2018, 50 (01) :29-38
[26]   Kam Sweet Rice (Oryza sativa L.) Is a Special Ecotypic Rice in Southeast Guizhou, China as Revealed by Genetic Diversity Analysis [J].
Liu, Chunhui ;
Cui, Di ;
Jiao, Aixia ;
Ma, Xiaoding ;
Li, Xiaobing ;
Han, Bing ;
Chen, Huicha ;
Ruan, Renchao ;
Wang, Yanjie ;
Han, Longzhi .
FRONTIERS IN PLANT SCIENCE, 2022, 13
[27]   Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method [J].
Livak, KJ ;
Schmittgen, TD .
METHODS, 2001, 25 (04) :402-408
[28]   Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2 [J].
Love, Michael I. ;
Huber, Wolfgang ;
Anders, Simon .
GENOME BIOLOGY, 2014, 15 (12)
[29]   Characteristic Flavor Compounds and Functional Components of Fragrant Rice with Different Flavor Types [J].
Lu, Lin ;
Hu, Zhanqiang ;
Fang, Changyun ;
Hu, Xianqiao .
FOODS, 2023, 12 (11)
[30]   Exogenous proline induces regulation in 2-acetyl-1-pyrroline (2-AP) biosynthesis and quality characters in fragrant rice (Oryza sativa L.) [J].
Luo, Haowen ;
Zhang, Tantan ;
Zheng, Axiang ;
He, Longxin ;
Lai, Rifang ;
Liu, Jinhai ;
Xing, Pipeng ;
Tang, Xiangru .
SCIENTIFIC REPORTS, 2020, 10 (01)