Chitosan-collagen-cerium hydroxyapatite nanocomposites for In-vitro gentamicin drug delivery and antibacterial properties

The controlled release of antibiotics is crucial to improving antimicrobial efficacy, reducing the risk of bacterial resistance, and ensuring a localized therapeutic effect. In this work, In-vitro Gentamicin release was studied using fluorescence chitosan collagen-cerium hydroxyapatite nanocomposites. Cerium-hydroxyapatite nanoparticles were synthesized using the hydrothermal method, and the nanocomposites were prepared by mixing chitosan-collagen-cerium hydroxyapatite at different weight ratios. Structural characterization was conducted using scanning electron microscopy, transmission electron microscopy, Fourier transform infrared spectroscopy, Raman spectroscopy, and fluorescence microscopy. Ultraviolet-visible spectroscopy (UV-Vis) was used to quantify the release of gentamicin in simulated body fluid. Results showed that hydroxyapatite releases 90 % of gentamicin in the first 10 min, and the Chitosan-collagen-cerium hydroxyapatite nanocomposites release 80 % of gentamicin after 2 h. The antibacterial activity was studied against Escherichia coli (E. coli) at different time intervals. These nanocomposites can potentially improve the performance of biomedical applications.