Study of the cellular and humoral immune responses to SARS-CoV-2 vaccination

被引：0

作者：

Montmaneix-Engels, Faustine ^{[1
,2
]}

Dimeglio, Chloe ^{[1
,3
]}

Staes, Laeticia ^{[3
]}

Da Silva, Isabelle ^{[3
]}

Porcheron, Marion ^{[3
]}

Jougla, Isabelle ^{[4
]}

Herin, Fabrice ^{[4
,5
]}

Izopet, Jacques ^{[1
,2
,3
]}

机构：

[1] Univ Toulouse III, CNRS, UMR5051, INSERM,UMR1291,Toulouse Inst Infect & Inflammatory, F-31300 Toulouse, France

[2] Toulouse III Paul Sabatier Univ, F-31062 Toulouse, France

[3] CHU Toulouse, Purpan Hosp, Virol Lab, F-31300 Toulouse, France

[4] Toulouse Univ Hosp, Occupat Dis Dept, F-31000 Toulouse, France

[5] Ctr Epidemiol & Res Populat Hlth Unit CERPOP, Joint Res Unit INSERM Univ Toulouse III Paul Sabat, UMR1295, F-31000 Toulouse, France

来源：

HELIYON | 2024年 / 10卷 / 07期

关键词：

COVID-19; vaccination; SARS-CoV-2; Cellular immunity; Humoral immunity; Interferon gamma release assay; BNT162B2;

D O I：

10.1016/j.heliyon.2024.e29116

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Our understanding of cellular immunity in response to COVID-19 infection or vaccination is limited because of less commonly used techniques. We investigated both the cellular and humoral immune responses before and after the administration of a third dose of the SARS-CoV-2 vaccine among a group of healthcare workers. Cellular immunity was evaluated using the VIDAS interferon-gamma (IFN gamma) RUO test, which enables automated measurement of IFN gamma levels after stimulating peripheral blood lymphocytes. Booster doses significantly enhanced both cellular and humoral immunity. Concerning cellular response, the booster dose increased the percentage of positive IFN gamma release assay (IGRA) results but no difference in IFN gamma release was found. The cellular response was not associated with protection against SARS-CoV-2 infection. Interestingly, vaccinated and infected healthcare workers exhibited the highest levels of anti-spike and neutralizing antibodies. In conclusion, the IGRA is a simple method for measuring cellular immune responses after vaccination. However, its usefulness as a complement to the study of humoral responses is yet to be demonstrated in future research.

引用

页数：8

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