Community coalescence and plant host filtering determine the spread of tetracycline resistance genes from pig manure into the microbiome continuum of the soil-plant system

被引:1
作者
Wen, Xin [1 ,2 ,3 ]
Xu, Jiaojiao [1 ]
Wang, Yiting [1 ]
Yang, Xin [1 ]
Peng, Guoliang [2 ]
Li, Siming [8 ]
Ma, Baohua [9 ]
Zou, Yongde [9 ]
Liao, Xindi [1 ,5 ,6 ,7 ]
Wang, Yan [1 ,5 ,6 ,7 ]
Worrich, Anja [3 ]
Wu, Yinbao [1 ,4 ,5 ,6 ,7 ,10 ]
机构
[1] South China Agr Univ, Coll Anim Sci, Coll Nat Resources & Environm, Guangdong Lab Lingnan Modern Agr, Guangzhou 510642, Peoples R China
[2] Shaoguan Univ, Guangdong Prov Key Lab Utilizat & Conservat Food &, Shaoguan 512005, Peoples R China
[3] UFZ Helmholtz Ctr Environm Res, Dept Environm Microbiol, D-04318 Leipzig, Germany
[4] Guangdong Lab Lingnan Modern Agr, Maoming Branch, Guangzhou 525000, Guangdong, Peoples R China
[5] South China Agr Univ, Guangdong Prov Key Lab Agroanim Genom & Mol Breedi, Minist Agr & Rural Affair, Guangzhou 510642, Peoples R China
[6] South China Agr Univ, Key Lab Chicken Genet Breeding & Reprod, Minist Agr & Rural Affair, Guangzhou 510642, Peoples R China
[7] South China Agr Univ, Coll Anim Sci, State Key Lab Swine & Poultry Breeding Ind, Guangzhou 510642, Peoples R China
[8] Shimadzu China Co LTD, Guangzhou Branch, Guangzhou 510656, Peoples R China
[9] Foshan Customs Comprehens Technol Ctr, Foshan 528200, Peoples R China
[10] South China Agr Univ, Coll Anim Sci, Natl Engn Res Ctr Breeding Swine Ind, Guangdong Lab Lingnan Modern Agr, Guangzhou 510642, Peoples R China
基金
中国国家自然科学基金;
关键词
Bacterial community; Antibiotic resistance genes; Animal manure; Soil-A. thaliana system; Doxycycline; ANTIBIOTIC-RESISTANCE; FERTILIZATION; PHYLLOSPHERE; DOXYCYCLINE;
D O I
10.1016/j.micres.2024.127734
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The spread of livestock manure-borne antibiotic resistance genes (ARGs) into agroecosystems through manure application poses a potential threat to human health. However, there is still a knowledge gap concerning ARG dissemination in coalescing manure, soil and plant microbiomes. Here, we examined the fate of tetracycline resistance genes (TRGs) originating from pig manure microbiomes and spread in the soil-A thaliana system and explored the effects of microbial functions on TRGs spread at different interfaces. Our results indicate that the TRGs abundances in all microbiome continuum of the soil-A. thaliana system were significantly increased with the application of a living manure microbiome, although the addition of manure with both an active and inactive microbiome caused a shift in the microbial community composition. This was attributed to the increasing relative abundances of tetA, tetL, tetM, tetO, tetW and tolC in the system. The application of living manure with DOX residues resulted in the highest relative abundance of total TRGs (3.30x10- 3 copies/16S rRNA gene copies) in the rhizosphere soil samples. Community coalescence of the manure and soil microbiomes increased the abundance of Firmicutes in the soil and root microbiome, which directly explains the increase in TRG abundance observed in these interfaces. In contrast, the leaf microbiome differed markedly from that of the remaining samples, indicating strong plant host filtering effects on Firmicutes and TRGs from pig manure. The random forest machine learning model revealed microbial functions and their significant positive correlation with TRG abundance in the microbiome continuum of the system. Our findings revealed that community coalescence is the main driver of TRG spread from manure to the soil and root microbiomes. Plant host filtering effects play a crucial role in allowing certain microbial groups to occupy ecological niches in the leaves, thereby limiting the establishment of manure-borne TRGs in aboveground plant tissues.
引用
收藏
页数:11
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