Biophysical aspects of migrasome organelle formation and their diverse cellular functions

The transient cellular organelles known as migrasomes, which form during cell migration along retraction fibers, have emerged as a crutial factor in various fundamental cellular processes and pathologies. These membrane vesicles originate from local membrane swellings, encapsulate specific cytoplasmic content, and are eventually released to the extracellular environment or taken up by recipient cells. Migrasome biogenesis entails a sequential membrane remodeling process involving a complex interplay between various molecular factors such as tetraspanin proteins, and mechanical properties like membrane tension and bending rigidity. In this review, we summarize recent studies exploring the mechanism of migrasome formation. We emphasize how physical forces, together with molecular factors, shape migrasome biogenesis, and detail the involvement of migrasomes in various cellular processes and pathologies. A comprehensive understanding of the exact mechanism underlying migrasome formation and the identification of key molecules involved hold promise for advancing their therapeutic and diagnostic applications. The biogenesis of migrasomes, organelles that develop from retraction fibers during cell migration, is regulated by biomolecules that modulate cellular physical factors such as cell adhesion, membrane tension, curvature and bending rigidity. This review discusses recent fundamental studies exploring the mechanism of migrasome formation and their potential implications for therapy and diagnosis. image