Effects of human umbilical cord mesenchymal stem cell-derived exosomes in the rat osteoarthritis models

被引：17

作者：

Yang, Huanfeng ^{[1
,2
]}

Zhou, Yiqin ^{[3
]}

Ying, Bi ^{[2
]}

Dong, Xuhui ^{[2
]}

Qian, Qirong ^{[3
]}

Gao, Shaorong ^{[1
,4
,5
]}

机构：

[1] Tongji Univ, Sch Life Sci & Technol, Shanghai Key Lab Signaling & Dis Res, Inst Regenerat Med,Shanghai East Hosp, Shanghai 200120, Peoples R China

[2] Oricell Therapeut, Dept R&D, Shanghai 201203, Peoples R China

[3] Naval Med Univ, Shanghai Changzheng Hosp, Dept Orthoped, Shanghai 200003, Peoples R China

[4] Tongji Univ, Shanghai Matern & Infant Hosp 1, Shanghai Inst Maternal Fetal Med & Gynecol Oncol, Clin & Translat Res Ctr,Shanghai Key Lab Maternal, Shanghai 201204, Peoples R China

[5] Tongji Univ, Frontier Sci Ctr Stem Cell Res, Shanghai 200092, Peoples R China

来源：

STEM CELLS TRANSLATIONAL MEDICINE | 2024年 / 13卷 / 08期

关键词：

mesenchymal stem cells; exosomes; osteoarthritis; umbilical cord; regeneration; KNEE OSTEOARTHRITIS; CARTILAGE; PATHOGENESIS; INFLAMMATION; CYTOKINES; PATHWAY; IL-6;

D O I：

10.1093/stcltm/szae031

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Mesenchymal stem cells (MSCs) offer great potential for treatment of osteoarthritis (OA) by promoting articular cartilage regeneration via paracrine secretion of exosomes; however, the underlying mechanisms are not fully understood. This study aimed to explore the therapeutic effects of exosomes secreted by human umbilical cord-derived MSCs (hUC-MSCs) in rat models of OA and reveal the underlying mechanisms. UC-MSCs and UC-MSC-exosomes were prepared and identified by transmission electron microscopy and flow cytometry. IL-1 beta-induced OA chondrocytes and the operation and collagenase-induced OA rat models were established. The results of micro-computed tomography, histology, and immunohistochemistry showed that UC-MSC-exosomes promoted cartilage regeneration in OA rats. ELISA results showed that the levels of synovial fluid cytokines, TNF-alpha, IL-1 beta, and IL-6, were lower in exosome therapy group than control group in both OA rat models. Exosome treatment significantly downregulated the expression of MMP-13 and ADAMTS-5 in chondrocytes stimulated by IL-1 beta, and upregulated collagen II expression. These findings suggest that hUC-MSC-exosomes offer a promising option for the therapy for OA. Graphical AbstractExosomes secreted by human umbilical cord-derived mesenchymal stem cells promoted cartilage regeneration in OA rats. The levels of synovial fluid cytokines (TNF-alpha, IL-1 beta, and IL-6) were significantly reduced in the synovial fluid of exosome treated group, and exosome treatment also significantly downregulated the expression of MMP-13 and ADAMTS-5 in chondrocytes stimulated by IL-1 beta.

引用

页码：803 / 811

页数：9

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