Evaluation of immune modulation activity, antioxidant activity and phytochemical screening of Croton bonplandianum Baill.

Croton bonplandianum Baill. is in the Euphorbiaceae family. Medicinal herbs offer anticancer, antioxidant, anti-inflammatory, and wound healing properties. We have decided to work in this innovative area. This was accomplished by prelude phytochemical analysis, LC-MS analysis, immunological modulation, and antioxidant activity of C. bonplandianum ethanolic extract (CBEE). Preliminary phytochemical research indicated the being there of phytochemicals such as sugars, quinines, and glycosides in C. bonplandianum leaf extract. The total flavonoids and phenolics content in leaf extracts were measured spectrophotometrically. The total phenolics content and total flavonoids content were resolute near are present 254.58 g/ml and 59.35 g/ml, respectively. LC-MS analysis was used to analyze the chemical composition of the ethanol of C. bonplandianum leaf extract. Using the LC-MS approach, we effectively detected key chemicals such as quercetin, phytol, kaempferol, and trehalose. The protective antioxidant capacity of C. bonplandianum leaves was determined using the DPPH test. The concentration of C. bonplandianum extract increased significantly, and the inhibitory concentration (IC50) value was found to be 57.05 g/ml.The cytotoxicity of the extract was assessed on the THP-1 cell line (a model for evaluating immunological modulation activities) using the MTT test. The cytotoxicity activity was moderate, with an IC50 of 213.5 +/- 0.084 mu g/ml. Protein profiling was analyzed with ELISA-IL4. The C. bonplandianum ethanolic extract significantly decreased IL-4 inflammatory cytokine production compared to the control (64.58 pg/ml or .00006458 mu g/ml). Cells treated with the IC50/ 2 dose of the sample showed a decrease of IL-4 inflammatory cytokine production 0.82 times (53.33 pg/ml or .00005333 mu g/ml).