Moderate amounts of selenium supplementation have a positive effect on the immune response. Selenium deficiency status can lead to reduced immunity, but the benefits of a natural selenium-rich diet for selenium deficiency are unknown. In this experiment, low selenium feed (0.02 mg Se/kg diet) was given to the mice for 5 weeks and selenium supplementation with selenoprotein (SeP) for 4 weeks, and the selenium-deficient mouse model was successfully established. SeP supplementation had significant protective effects, including increased antioxidant capacity and alleviation of inflammatory damage. SeP markedly elevated the activities of thioredoxin reductase (TrxR) and glutathione peroxidase (GSH-Px) in the serum, kidney, liver, and spleen of seleniumdeficient mice. SeP can restore the condition of damaged spleen tissue, raise the spleen index in mice, and encourage an increase in immunoglobulin (IgA, IgG, and IgM) content. The levels of catalase (CAT), total antioxidant capacity (T-AOC), GSH-Px, and cytokines (IL-1 beta, IL-6, and TNF-alpha) were all significantly elevated by SeP. SeP showed superior immunomodulatory effects over inorganic selenium (Na2SeO3). Together, these results suggest that SeP may be a potential dietary selenium supplement that can reduce symptoms of spleen damage caused by selenium deficiency.
