Molecular characterization of heat shock protein 70 gene in Deoni cattle ( Bos indicus )

The present study was conducted in 60 Deoni cattle raised under semi -arid, hot tropical climatic conditions of Marathwada region of Maharashtra with the objectives to identify single nucleotide polymorphism (SNPs) in targeted promoter regions of HSP70 gene and to analyse the association of different genotypes with physiological parameters. The PCR amplification was performed using primers available in the NCBI database and revealed size of 539 bp for the amplified fragment promoter region 1 of HSP70 gene. PCR-RFLP analysis with HindIII restriction enzyme of targeted primer produced two different banding patterns, resulting in their identification as homozygotes AA and BB genotypes. Sequence analysis revealed single nucleotide polymorphism in coding promoter region of HSP70 gene at 284 (th) position A to G, the A284G SNP resolved into two genotypes i.e. AA and BB, showed non -significant association with physiological parameters (rectal temperature and respiration rate) with a change in the amino acid from valine to isoleucine. However, there was no any change observed for physiological parameters in the studied population. Associations between HSP70 gene polymorphism in Deoni cattle with physiological parameter i.e. respiration rate and rectal temperature were analysed and rectal temperature in genotype AA was 100.52 +/- 0.023, in genotype BB it was 100.65 +/- 0.019. Similarly, 19.07 +/- 0.117 respiration rate in genotype AA while 19.17 +/- 0.108 in genotype BB were observed. The observed rectal temperature and respiration rate across different genotypes were found to be non- significantly (p >0.05) differ. Therefore, there is scope of considering genetic information of HSP70 gene in breeding and management strategies for the improvement of Deoni cattle by considering large number of Deoni population.