1α,25-dihydroxyvitamin D3-eluting nanofibrous dressings induce endogenous antimicrobial peptide expression

被引：19

作者：

Jiang, Jiang ^{[1
]}

Zhang, Yang ^{[2
,3
]}

Indra, Arup K. ^{[2
,4
,5
,6
]}

Ganguli-Indra, Gitali ^{[4
,6
]}

Le, Mai N. ^{[4
]}

Wang, Hongjun ^{[1
]}

Hollins, Ronald R. ^{[7
]}

Reilly, Debra A. ^{[7
]}

Carlson, Mark A. ^{[8
,9
]}

Gallo, Richard L. ^{[10
]}

Gombart, Adrian F. ^{[2
,3
]}

Xie, Jingwei ^{[1
]}

机构：

[1] Univ Nebraska Med Ctr, Dept Surg, Transplant & Holland Regenerat Med Program, Omaha, NE 68198 USA

[2] Oregon State Univ, Linus Pauling Inst, Dept Biochem & Biophys, Corvallis, OR 97331 USA

[3] Oregon State Univ, Coll Publ Hlth & Human Sci, Sch Biol & Populat Hlth Sci, Nutr Grad Program, Corvallis, OR 97331 USA

[4] Oregon State Univ, Coll Pharm, Dept Pharmaceut Sci, Corvallis, OR 97331 USA

[5] OHSU, Dept Dermatol, Portland, OR 97239 USA

[6] OHSU, Knight Canc Inst, Portland, OR 97239 USA

[7] Univ Nebraska Med Ctr, Dept Surg Plast & Reconstruct Surg, Omaha, NE 68198 USA

[8] Univ Nebraska Med Ctr, Dept Surg Gen Surg & Genet Cell Biol & Anat, Omaha, NE 68198 USA

[9] VA Nebraska Western Iowa Hlth Care Syst, Dept Surg, Omaha, NE 68105 USA

[10] Univ Calif San Diego, Dept Dermatol, San Diego, CA 92093 USA

来源：

NANOMEDICINE | 2018年 / 13卷 / 12期

关键词：

1; alpha; 25-dihydroxyvitamin D-3; antimicrobial peptides LL-37; electrospun nanofibers; endogenous production; innate immunity; sustained delivery; SURGICAL SITE INFECTION; VITAMIN-D-RECEPTOR; GLIOMA IN-VITRO; WOUND DRESSINGS; 1,25-DIHYDROXYVITAMIN D-3; ELECTROSPUN NANOFIBERS; SUSTAINED DELIVERY; GENE-EXPRESSION; HOST RESPONSE; HUMAN SKIN;

D O I：

10.2217/nnm-2018-0011

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Aim: The aim of this study was to develop a nanofiber-based dressing capable of local sustained delivery of 1 alpha,25-dihydroxyvitamin D-3 (1,25(OH)(2)D-3) and augmenting human CAMP induction. Materials & methods: Nanofibrous wound dressings containing 1,25(OH)(2)D-3 were successfully prepared by electrospinning, which were examined in vitro, in vivo and ex vivo. Results: 1,25(OH)(2)D-3 was successfully loaded into nanofibers with encapsulation efficiency larger than 90%. 1,25(OH)(2)D-3 showed a sustained release from nanofibers over 4 weeks. Treatment of U937 and HaCaT cells with 1,25(OH)(2)D-3-loaded poly(epsilon-caprolactone) nanofibers significantly induced hCAP18/LL37 expression in monocytes and keratinocytes, skin wounds of humanized transgenic mice and artificial wounds of human skin explants. Conclusion: 1,25(OH)(2)D-3 containing nanofibrous dressings could enhance innate immunity by inducing antimicrobial peptide production.

引用

页码：1417 / 1432

页数：16

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