Insect Cell-Based Quadrivalent Seasonal Influenza Virus-like Particles Vaccine Elicits Potent Immune Responses in Mice

被引:0
作者
Badruzzaman, A. T. M. [1 ,2 ]
Cheng, Yu-Chieh [1 ]
Sung, Wang-Chou [1 ]
Lee, Min-Shi [1 ]
机构
[1] Natl Hlth Res Inst NHRI, Natl Inst Infect Dis & Vaccinol, 35 Keyan Rd, Zhunan 350, Taiwan
[2] Natl Cent Univ, Dept Life Sci, Taoyuan 320, Taiwan
关键词
seasonal influenza; virus-like particle (VLP); baculovirus expression system; vaccine; ADULTS; 18-49; YEARS; PROTECTIVE IMMUNITY; IMMUNOGENICITY; RECOMBINANT; INFECTION; SAFETY; TRIAL; ASSAY;
D O I
10.3390/vaccines12060667
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Influenza viruses can cause highly infectious respiratory diseases, posing noteworthy epidemic and pandemic threats. Vaccination is the most cost-effective intervention to prevent influenza and its complications. However, reliance on embryonic chicken eggs for commercial influenza vaccine production presents potential risks, including reductions in efficacy due to HA gene mutations and supply delays due to scalability challenges. Thus, alternative platforms are needed urgently to replace egg-based methods and efficiently meet the increasing demand for vaccines. In this study, we employed a baculovirus expression vector system to engineer HA, NA, and M1 genes from seasonal influenza strains A/H1N1, A/H3N2, B/Yamagata, and B/Victoria, generating virus-like particle (VLP) vaccine antigens, H1N1-VLP, H3N2-VLP, Yamagata-VLP, and Victoria-VLP. We then assessed their functional and antigenic characteristics, including hemagglutination assay, protein composition, morphology, stability, and immunogenicity. We found that recombinant VLPs displayed functional activity, resembling influenza virions in morphology and size while maintaining structural integrity. Comparative immunogenicity assessments in mice showed that our quadrivalent VLPs were consistent in inducing hemagglutination inhibition and neutralizing antibody titers against homologous viruses compared to both commercial recombinant HA and egg-based vaccines (Vaxigrip). The findings highlight insect cell-based VLP vaccines as promising candidates for quadrivalent seasonal influenza vaccines. Further studies are worth conducting.
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页数:15
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