Characterization of pain-related behaviors in a rat model of acute-to-chronic low back pain: single vs. multi-level disc injury

被引：6

作者：

Barbe, Mary F. ^{[1
]}

Chen, Frank Liu ^{[1
]}

Loomis, Regina H. ^{[1
]}

Harris, Michele Y. ^{[1
]}

Kim, Brandon M. ^{[2
]}

Xie, Kevin ^{[1
]}

Hilliard, Brendan A. ^{[1
]}

McGonagle, Elizabeth R. ^{[1
]}

Bailey, Taylor D. ^{[1
]}

Gares, Ryan P. ^{[1
]}

Van der Bas, Megan ^{[1
]}

Kalicharan, Betsy A. ^{[1
]}

Holt-Bright, Lewis ^{[1
]}

Stone, Laura S. ^{[3
]}

Hodges, Paul W. ^{[4
]}

Klyne, David M. ^{[4
]}

机构：

[1] Temple Univ, Aging Cardiovasc Discovery Ctr, Lewis Katz Sch Med, Philadelphia, PA 19122 USA

[2] Temple Univ, Lewis Katz Sch Med, Med Doctor Program, Philadelphia, PA USA

[3] Univ Minnesota, Dept Anesthesiol, Minneapolis, MN USA

[4] Univ Queensland, NHMRC Ctr Clin Res Excellence Spinal Pain Injury &, Sch Hlth & Rehabil Sci, Brisbane, Qld, Australia

来源：

FRONTIERS IN PAIN RESEARCH | 2024年 / 5卷

关键词：

low back pain; acute to chronic; disc puncture; algometer; von frey; social interaction skills; open field; pain; TUMOR-NECROSIS-FACTOR; NERVE GROWTH-FACTOR; INTERVERTEBRAL DISC; NEUROTROPHIC FACTOR; FACTOR-ALPHA; CENTRAL SENSITIZATION; TNF-ALPHA; PROINFLAMMATORY CYTOKINES; GLOBAL BURDEN; HERNIATION;

D O I：

10.3389/fpain.2024.1394017

中图分类号：

R74 [神经病学与精神病学];

学科分类号：

摘要：

Introduction Low back pain is the most common type of chronic pain. We examined pain-related behaviors across 18 weeks in rats that received injury to one or two lumbar intervertebral discs (IVD) to determine if multi-level disc injuries enhance/prolong pain.Methods Twenty-three Sprague-Dawley adult female rats were used: 8 received disc puncture (DP) of one lumbar IVD (L5/6, DP-1); 8 received DP of two lumbar IVDs (L4/5 & L5/6, DP-2); 8 underwent sham surgery.Results DP-2 rats showed local (low back) sensitivity to pressure at 6- and 12-weeks post-injury, and remote sensitivity to pressure (upper thighs) at 12- and 18-weeks and touch (hind paws) at 6, 12 and 18-weeks. DP-1 rats showed local and remote pressure sensitivity at 12-weeks only (and no tactile sensitivity), relative to Sham DP rats. Both DP groups showed reduced distance traveled during gait testing over multiple weeks, compared to pre-injury; only DP-2 rats showed reduced distance relative to Sham DP rats at 12-weeks. DP-2 rats displayed reduced positive interactions with a novel adult female rat at 3-weeks and hesitation and freezing during gait assays from 6-weeks onwards. At study end (18-weeks), radiological and histological analyses revealed reduced disc height and degeneration of punctured IVDs. Serum BDNF and TNF alpha levels were higher at 18-weeks in DP-2 rats, relative to Sham DP rats, and levels correlated positively with remote sensitivity in hind paws (tactile) and thighs (pressure).Discussion Thus, multi-level disc injuries resulted in earlier, prolonged and greater discomfort locally and remotely, than single-level disc injury. BDNF and TNF alpha may have contributing roles.

引用

页数：23

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