Brahma is required for cell cycle arrest and late muscle gene expression during skeletal myogenesis

被引:29
作者
Albini, Sonia [1 ]
Toto, Paula Coutinho [1 ]
Dall'Agnese, Alessandra [1 ]
Malecova, Barbora [1 ]
Cenciarelli, Carlo [2 ]
Felsani, Armando [3 ]
Caruso, Maurizia [3 ]
Bultman, Scott J. [4 ]
Puri, Pier Lorenzo [1 ,5 ]
机构
[1] Sanford Burnham Inst Med Res, La Jolla, CA 92037 USA
[2] CNR Ist Farmacol Traslaz, Rome, Italy
[3] Fdn Santa Lucia, CNR Ist Biol Cellulare & Neurobiol, Rome, Italy
[4] Univ N Carolina, Lineberger Comprehens Canc Ctr, Dept Genet, Chapel Hill, NC 27599 USA
[5] IRCCS Fdn Santa Lucia, Rome, Italy
基金
美国国家卫生研究院;
关键词
Brahma; cyclin D1; skeletal myogenesis; SNF/SWI; transcription; CHROMATIN-REMODELING COMPLEXES; MAMMALIAN SWI/SNF COMPLEXES; SATELLITE CELL; TRANSCRIPTIONAL ACTIVATION; RETINOBLASTOMA PROTEIN; ATPASE BRG1; STEM-CELLS; DIFFERENTIATION; WITHDRAWAL; MUTATION;
D O I
10.15252/embr.201540159
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Although the two catalytic subunits of the SWI/SNF chromatin-remodeling complex-Brahma (Brm) and Brg1-are almost invariably co-expressed, their mutually exclusive incorporation into distinct SWI/SNF complexes predicts that Brg1-and Brm-based SWI/SNF complexes execute specific functions. Here, we show that Brg1 and Brm have distinct functions at discrete stages of muscle differentiation. While Brg1 is required for the activation of muscle gene transcription at early stages of differentiation, Brm is required for Ccnd1 repression and cell cycle arrest prior to the activation of muscle genes. Ccnd1 knockdown rescues the ability to exit the cell cycle in Brm-deficient myoblasts, but does not recover terminal differentiation, revealing a previously unrecognized role of Brm in the activation of late muscle gene expression independent from the control of cell cycle. Consistently, Brm null mice displayed impaired muscle regeneration after injury, with aberrant proliferation of satellite cells and delayed formation of new myofibers. These data reveal stage-specific roles of Brm during skeletal myogenesis, via formation of repressive and activatory SWI/SNF complexes.
引用
收藏
页码:1037 / 1050
页数:14
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