LncRNA ADAMTS9-AS1 inhibits the stemness of lung adenocarcinoma cells by regulating miR-5009-3p/NPNT axis

被引：9
作者：
Wang, Ping [1 ,2 ]
Zhang, Yanli [1 ,2 ]
Lv, Xiaodong [2 ]
Zhou, Jian [1 ,2 ]
Cang, Shundong [3 ]
Song, Yongping [1 ,2 ]
机构：
[1] Zhengzhou Univ, Dept Hematol, Affiliated Canc Hosp, 127 Dongming Rd, Zhengzhou 450008, Henan, Peoples R China
[2] Henan Canc Hosp, 127 Dongming Rd, Zhengzhou 450008, Henan, Peoples R China
[3] Zhengzhou Univ, Affiliated Canc Hosp, Cent Lab, Zhengzhou 450008, Henan, Peoples R China
来源：
GENOMICS | 2023年 / 115卷 / 03期
关键词：
Cancer stem cell; Stemness; Lung adenocarcinoma; Colony formation; miR-5009-3p; PROLIFERATION; CANCER; PROGRESSION; MIGRATION; INVASION; EMT;
D O I：
10.1016/j.ygeno.2023.110596
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
We sought to extend our observation of LncRNA ADAMTS9-AS1 and to specifically uncover its role on the stemness of lung adenocarcinoma (LUAD) cancer cells. ADAMTS9-AS1 was poorly expressed in LUAD. The high ADAMTS9-AS1 expression was positively associated with overall survival. ADAMTS9-AS1 overexpression attenuated the colony-forming capacity and reduced stem cell-like population of LUAD cancer stem cells (CSCs). Furthermore, ADAMTS9-AS1 overexpression increased E-cadherin expression in addition to the downregulated expressions of Fibronectin and Vimentin in LUAD spheres. In vitro results also confirmed the ADAMTS9-AS1's inhibitory effect on the growth of LUAD cells. Moreover, the antagonistic repression of miR-5009-3p levels with the expression of ADAMTS9-AS1 and NPNT was confirmed. Finally, ADAMTS9-AS1 overexpression curbed the increasing stemness of LUDA-CSC caused by NPNT silencing, thus leading to the suppression of LUAD progression in vitro. Conclusively, ADAMTS9-AS1 negatively controls the LUAD cancer cell stemness progression through regulating miR-5009-3p/NPNT axis.
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页数：11
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