Isolation and characterization of two Staphylococcus aureus lytic bacteriophages "Huma" and "Simurgh"

被引：7

作者：

Sharifi, Fatemeh ^{[1
]}

Montaseri, Maryam ^{[1
]}

Yousefi, Mohammad Hashem ^{[1
]}

Shekarforoush, Seyed Shahram ^{[1
]}

Berizi, Enayat ^{[2
]}

Wagemans, Jeroen ^{[3
]}

Vallino, Marta ^{[4
]}

Hosseinzadeh, Saeid ^{[1
]}

机构：

[1] Shiraz Univ, Sch Vet Med, Dept Food Hyg & Publ Hlth, Shiraz, Iran

[2] Shiraz Univ Med Sci, Sch Nutr & Food Sci, Dept Food Hyg & Qual Control, Shiraz, Iran

[3] Katholieke Univ Leuven, Dept Biosyst, B-3001 Leuven, Belgium

[4] Natl Res Council Italy, Inst Sustainable Plant Protect, I-10135 Turin, Italy

来源：

VIROLOGY | 2024年 / 595卷

关键词：

Bacteriophage; Rosenblumvirus; Staphylococcus aureus; Lytic phage; PHAGES;

D O I：

10.1016/j.virol.2024.110090

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Nowadays finding the new antimicrobials is necessary due to the emerging of multidrug resistant strains. The present study aimed to isolate and characterize bacteriophages against S. aureus. Strains Huma and Simurgh were the two podovirus morphology phages which isolated and then characterized. Huma and Simurgh had a genome size of 16,853 and 17,245 bp, respectively and both were Rosenblumvirus with G + C content of 29%. No lysogeny-related genes, nor virulence genes were identified in their genomes. They were lytic only against two out of four S. aureus strains. They also were able to inhibit S. aureus for 8 h in-vitro. Both showed a rapid adsorption. Huma and Simurgh had the latent period of 80 and 60 m and the burst sizes of 45 and 40 PFU/ml and also, they showed very low cell toxicity of 1.23%-1.79% on HT-29 cells, respectively. Thus, they can be considered potential candidates for biocontrol applications.

引用

页数：8

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