COMPARISON OF PERMANENT STAINING METHODS FOR THE LABORATORY DIAGNOSIS OF TRICHOMONIASIS

被引：2

作者：

Menezes, Camila Braz ^{[1
]}

Mello, Mariana dos Santos ^{[2
]}

Tasca, Tiana ^{[1
]}

机构：

[1] Univ Fed Rio Grande do Sul, Fac Farm, Lab Pesquisa Parasitol, Av Ipiranga 2752, BR-90610000 Porto Alegre, RS, Brazil

[2] Univ Fed Rio Grande do Sul, Fac Farm, Lab Anal Clin & Toxicol, Av Ipiranga 2752, BR-90610000 Porto Alegre, RS, Brazil

来源：

REVISTA DO INSTITUTO DE MEDICINA TROPICAL DE SAO PAULO | 2016年 / 58卷

关键词：

Trichomonas vaginalis; Diagnosis; Morphology; Fixing and staining techniques; PAPANICOLAOU SMEAR; VAGINALIS; INFECTION; CULTURE;

D O I：

10.1590/S1678-994620160005

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Trichomonas vaginalis is the etiologic agent of trichomoniasis, the most common non-viral sexually transmitted disease ( STD) in the world. The diagnosis is based on wet mount preparation and direct microscopy on fixed and stained clinical specimens. The aim of this study was to compare the performance of different fixing and staining techniques used in the detection of T. vaginalis in urine. The smears were fixed and submitted to different methods of permanent staining and then, the morphological aspects of the parasites were analyzed and compared. The Papanicolaou staining with ethanol as the fixative solution showed to be the best method of permanent staining. Our data suggest that staining techniques in association with wet mount examination of fresh specimens contribute to increase the sensitivity in the diagnosis of trichomoniasis.

引用

页数：4

共 25 条

[1]

[Anonymous], 2005, PROGR NAC DST AIDS P

[2]

CREE GE, 1968, BRIT J VENER DIS, V44, P226

[3] THE ESTABLISHMENT OF VARIOUS TRICHOMONADS OF ANIMALS AND MAN IN AXENIC CULTURES [J].