Azelaic acid can efficiently compete for the auxin binding site TIR1, altering auxin polar transport, gravitropic response, and root growth and architecture in Arabidopsis thaliana roots

被引：1

作者：

Alvarez-Rodriguez, Sara ^{[1
,2
]}

Araniti, Fabrizio ^{[3
]}

Teijeira, Marta ^{[4
,5
]}

Reigosa, Manuel J. ^{[1
,2
]}

Sanchez-Moreiras, Adela M. ^{[1
,2
]}

机构：

[1] Univ Vigo, Dept Biol Vexetal & Ciencias Solo, Fac Biol, Campus Lagoas Marcosende S-N, Vigo 36310, Spain

[2] Univ Vigo, Inst Agroecol & Alimentac IAA, Campus Auga, Orense 32004, Spain

[3] Univ Statale Milano, Dipartimento Sci Agr & Ambientali Prod Terr Agroen, Via Celoria 2, I-20133 Milan, Italy

[4] Univ Vigo, Dept Quim Organ, Fac Quim, Vigo 36310, Spain

[5] Hosp Alvaro Cunqueiro, Inst Invest Sanitaria Galicia, Vigo 36213, Spain

来源：

PLANT PHYSIOLOGY AND BIOCHEMISTRY | 2024年 / 210卷

关键词：

Azelaic acid; Auxin; Auxin polar transport; Gravitropism; TIR1; RESISTANCE; 2,4-D;

D O I：

10.1016/j.plaphy.2024.108592

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The present study investigates the phytotoxic potential of azelaic acid (AZA) on Arabidopsis thaliana roots. Effects on root morphology, anatomy, auxin content and transport, gravitropic response and molecular docking were analysed. AZA inhibited root growth, stimulated lateral and adventitious roots, and altered the root apical meristem by reducing meristem cell number, length and width. The treatment also slowed down the roots' gravitropic response, likely due to a reduction in statoliths, starch-rich organelles involved in gravity perception. In addition, auxin content, transport and distribution, together with PIN proteins' expression and localisation were altered after AZA treatment, inducing a reduction in auxin transport and its distribution into the meristematic zone. Computational simulations showed that AZA has a high affinity for the auxin receptor TIR1, competing with auxin for the binding site. The AZA binding with TIR1 could interfere with the normal functioning of the TIR1/AFB complex, disrupting the ubiquitin E3 ligase complex and leading to alterations in the response of the plant, which could perceive AZA as an exogenous auxin. Our results suggest that AZA mode of action could involve the modulation of auxin-related processes in Arabidopsis roots. Understanding such mechanisms could lead to find environmentally friendly alternatives to synthetic herbicides.

引用

页数：12