Preparation and characterization of kelp polysaccharide and its research on anti-influenza a virus activity

被引：2

作者：

Pi, Tianxiang ^{[1
]}

Sun, Lishan ^{[2
]}

Li, Wei ^{[3
]}

Wang, Wei ^{[2
]}

Dong, Minghui ^{[4
]}

Xu, Xinxing ^{[1
]}

Xu, He ^{[5
]}

Zhao, Yuanhui ^{[1
]}

机构：

[1] Ocean Univ China, Coll Food Sci & Engn, State Key Lab Marine Food Proc & Safety Control, Qingdao 266003, Peoples R China

[2] Ocean Univ China, Sch Med & Pharm, Qingdao 266003, Peoples R China

[3] Dist Hosp Qingdao West Coast New Area, Dept Gen Surg, Qingdao 266400, Peoples R China

[4] Zhongxin Anderson Guangdong Biotechnol Co Ltd, Guangzhou 510000, Peoples R China

[5] Lianyungang Baohong Marine Technol Co Ltd, Lianyungang 222000, Peoples R China

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2024年 / 280卷

基金：

中国国家自然科学基金;

关键词：

Kelp polysaccharide; Enzyme hydrolysis; Anti-influenza; Neuraminidase; STRUCTURAL-CHARACTERIZATION; MECHANISM;

D O I：

10.1016/j.ijbiomac.2024.135506

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The beneficial effects of kelp polysaccharide (KPS) have recently attracted attention. In this study, KPS was extracted from kelp using the enzyme hydrolysis combined with freeze-drying, namely, KPS-EF. The structural characterization showed that KPS-EF was a highly sulfated macromolecule with the Mw of 764.2 kDa and the sulfate content of 23.49 %. The antiviral activity of KPS-EF in vitro was verified, and the IC50 value of KPS against the PR8 virus was 0.58 mg/mL. Intranasal administration of KPS-EF significantly inhibited death and weight loss in IAV-infected mice and alleviated virus-induced pneumonia symptoms, meanwhile, KPS-EF (10 mg/kg/day) significantly decreased the production levels of chemokines (CXCL1, RANTES) and inflammatory cytokines (IL-6, TNF-alpha) in lungs (p < 0.05). KPS-EF could downregulate the activity of viral neuraminidase (NA) primarily in the late stage of viral adsorption with an IC50 value of 0.29 mg/mL. This study provides a theoretical basis for the using KPS as a supplement to NA inhibitors or anti-influenza drugs.

引用

页数：15

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