Construction and analysis of the immune effect of two different vaccine types based on Vibrio harveyi VgrG

被引:2
|
作者
Du, Xiangyu [1 ,2 ,3 ]
Kang, Minjie [2 ,3 ]
Yang, Chunhuan [2 ,3 ]
Yao, Xinping [2 ,3 ]
Zheng, Lvliang [1 ,2 ]
Wu, Ying [1 ,2 ,3 ]
Zhang, Panpan [1 ,2 ,3 ]
Zhang, Han [1 ,2 ,3 ]
Zhou, Yongcan [1 ,2 ,3 ]
Sun, Yun [1 ,2 ,3 ]
机构
[1] Hainan Univ, Sanya Inst Breeding & Multiplicat, Sch Breeding & Multiplicat, Sanya 572025, Peoples R China
[2] Hainan Univ, Collaborat Innovat Ctr Marine Sci & Technol, Haikou 570228, Peoples R China
[3] Hainan Univ, Sch Marine Biol & Fisheries, Haikou 570228, Peoples R China
关键词
Vibrio harveyi; VgrG; DNA vaccine; Subunit vaccine; NOCARDIA-SERIOLAE; DNA VACCINE; PROTEINS; FISH;
D O I
10.1016/j.fsi.2024.109494
中图分类号
S9 [水产、渔业];
学科分类号
0908 ;
摘要
Vibrio harveyi poses a significant threat to fish and invertebrates in mariculture, resulting in substantial financial repercussions for the aquaculture sector. Valine-glycine repeat protein G (VgrG) is essential for the type VI secretion system's (T6SS) assembly and secretion. VgrG from V. harveyi QT520 was cloned and analyzed in this study. The localization of VgrG was determined by Western blot, which revealed that it was located in the cytoplasm, secreted extracellularly, and attached to the membrane. The effectiveness of two vaccinations against V. harveyi infection-a subunit vaccine (rVgrG) and a DNA vaccine (pCNVgrG) prepared with VgrG was evaluated. The findings indicated that both vaccines provided a degree of protection against V. harveyi challenge. At 4 weeks post-vaccination (p.v.), the rVgrG and pCNVgrG exhibited relative percent survival rates (RPS) of 71.43% and 76.19%, respectively. At 8 weeks p.v., the RPS for rVgrG and pCNVgrG were 68.21% and 72.71%, respectively. While both rVgrG and pCNVgrG elicited serum antibody production, the subunit vaccinated fish demonstrated significantly higher levels of serum anti-VgrG specific antibodies than the DNA vaccine group. The result of qRT-PCR demonstrated that the expression of major histocompatibility complex (MHC) class I alpha, tumor necrosis factor-alpha (TNF-alpha), interferon gamma (IFN gamma), and cluster of differentiation 4 (CD4) were up-regulated by both rVgrG and pCNVgrG. Fish vaccinated with rVgrG and pCNVgrG exhibited increased activity of acid phosphatase, alkaline phosphatase, superoxide dismutase, and lysozyme. These findings suggest that VgrG from V. harveyi holds potential for application in vaccination.
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页数:8
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