The effect of Ca 2+-calcineurin signaling pathway on the antifungal activity of Pd-D-V against Botrytis cinerea

Gray mold, caused by Botrytis cinerea is an intractable fungal disease that causes extensive damage to agricultural products. In the search for novel antifungal active ingredients, we discovered a linear pyranocoumarin Pd-D-V was effective against B. cinerea in both in vitro and in vivo assays. Furthermore, this study investigated the effects of Ca 2 + and the Ca 2 +-calcineurin signaling pathway on its antifungal activity against B. cinerea . The results indicated that Pd-D-V reduced the concentration of Ca 2 + in the mycelia of B. cinerea ; CaCl 2 , the Ca 2 + channel blocker verapamil, or the calcineurin inhibitor cyclosporin A could affect the sensitivity of Pd-D-V against B. cinerea ; the expression of genes ( Bccch1, Bcmid1, BccnA, Bccnb1, Bcpmc1, and Bcpmr1 ) of the Ca 2 +-calcineurin signaling pathway decreased after Pd-D-V treatment. In summary, Pd-D-V is compound for developing fungicides against B. cinerea . Pd-D-V can reduce intracellular Ca 2 + concentration and disturb Ca 2 + homeostasis. The Ca 2 +- calcineurin signaling pathway is important in the antifungal activity of Pd-D-V against B. cinerea .