Antimicrobial Activity of Bovine Bone Scaffolds Impregnated with Silver Nanoparticles on New Delhi Metallo-β-Lactamase-Producing Gram-Negative Bacilli Biofilms

被引:1
作者
Goncalves, Geiziane Aparecida [1 ]
Ribeiro, Victoria Stadler Tasca [1 ]
Dantas, Leticia Ramos [1 ]
de Andrade, Ana Paula [1 ]
Suss, Paula Hansen [1 ]
Witt, Maria Alice [2 ]
Tuon, Felipe Francisco [1 ]
机构
[1] Pontificia Univ Catol Parana, Sch Med, Grad Program Hlth Sci, Lab Emerging Infect Dis, BR-80215901 Curitiba, PR, Brazil
[2] Pontificia Univ Catol Parana, Chem Dept, BR-80215901 Curitiba, PR, Brazil
来源
COMPOUNDS | 2023年 / 3卷 / 04期
关键词
biofilm; silver; bone; nanotechnology; impregnation; bacteria; NDM; scaffold; GOLD NANOPARTICLES; IN-VITRO; SUSCEPTIBILITY; RESISTANCE; INFECTION; TISSUE;
D O I
10.3390/compounds3040042
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Introduction: The antibiofilm activity of silver nanoparticles has been extensively investigated in common bacteria. Metallo-beta-lactamase-producing Gram-negative bacteria are hard-to-treat microorganisms with few therapeutic options, and silver nanoparticles were not evaluated on the biofilm of these bacteria. Objectives: The aim of this study was to evaluate the antibiofilm activity of a bone scaffold impregnated with silver nanoparticles in NDM-producing Gram-negative bacilli. Methods: Bone scaffolds from bovine femur were used for the tests and impregnated with silver nanoparticles (50 nm) by physical adsorption. Silver nitrate minimal inhibitory and bactericidal concentrations (MIC and MBC) were performed on NDM-producing Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa. Disc diffusion tests for silver nanoparticles' susceptibility and the quantification of biofilm production on plate and bone with sessile cell count were performed. Results: The MIC results demonstrated that silver nitrate had an antimicrobial effect on all microorganisms, inactivating the growth of isolates from a concentration of 8 mu g/mL. MBC results showed that E. coli 16.211 was the only isolate to present MIC that were different from MBC, with a value of 16 mu g/mL. Conclusion: Bone scaffolds impregnated with silver nanoparticles can significantly reduce the biofilm of multidrug-resistant bacteria. This is a strategical material that can be used as bone implant in different clinical conditions.
引用
收藏
页码:584 / 595
页数:12
相关论文
共 62 条
  • [61] Quantitative Proteomics Reveals the Mechanism of Silver Nanoparticles against Multidrug-Resistant Pseudomonas aeruginosa Biofilms
    Zhang, Yapeng
    Pan, Xuanhe
    Liao, Shijing
    Jiang, Congyuan
    Wang, Linqian
    Tang, Yurong
    Wu, Guojun
    Dai, Gan
    Chen, Liyu
    [J]. JOURNAL OF PROTEOME RESEARCH, 2020, 19 (08) : 3109 - 3122
  • [62] Prosthetic-joint-associated infections
    Zimmerli, Werner
    [J]. BEST PRACTICE & RESEARCH IN CLINICAL RHEUMATOLOGY, 2006, 20 (06): : 1045 - 1063