Antimicrobial Activity of Bovine Bone Scaffolds Impregnated with Silver Nanoparticles on New Delhi Metallo-β-Lactamase-Producing Gram-Negative Bacilli Biofilms
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Goncalves, Geiziane Aparecida
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Pontificia Univ Catol Parana, Sch Med, Grad Program Hlth Sci, Lab Emerging Infect Dis, BR-80215901 Curitiba, PR, BrazilPontificia Univ Catol Parana, Sch Med, Grad Program Hlth Sci, Lab Emerging Infect Dis, BR-80215901 Curitiba, PR, Brazil
Goncalves, Geiziane Aparecida
[1
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Ribeiro, Victoria Stadler Tasca
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Pontificia Univ Catol Parana, Sch Med, Grad Program Hlth Sci, Lab Emerging Infect Dis, BR-80215901 Curitiba, PR, BrazilPontificia Univ Catol Parana, Sch Med, Grad Program Hlth Sci, Lab Emerging Infect Dis, BR-80215901 Curitiba, PR, Brazil
Ribeiro, Victoria Stadler Tasca
[1
]
Dantas, Leticia Ramos
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Pontificia Univ Catol Parana, Sch Med, Grad Program Hlth Sci, Lab Emerging Infect Dis, BR-80215901 Curitiba, PR, BrazilPontificia Univ Catol Parana, Sch Med, Grad Program Hlth Sci, Lab Emerging Infect Dis, BR-80215901 Curitiba, PR, Brazil
Dantas, Leticia Ramos
[1
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de Andrade, Ana Paula
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Pontificia Univ Catol Parana, Sch Med, Grad Program Hlth Sci, Lab Emerging Infect Dis, BR-80215901 Curitiba, PR, BrazilPontificia Univ Catol Parana, Sch Med, Grad Program Hlth Sci, Lab Emerging Infect Dis, BR-80215901 Curitiba, PR, Brazil
de Andrade, Ana Paula
[1
]
Suss, Paula Hansen
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Pontificia Univ Catol Parana, Sch Med, Grad Program Hlth Sci, Lab Emerging Infect Dis, BR-80215901 Curitiba, PR, BrazilPontificia Univ Catol Parana, Sch Med, Grad Program Hlth Sci, Lab Emerging Infect Dis, BR-80215901 Curitiba, PR, Brazil
Suss, Paula Hansen
[1
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Witt, Maria Alice
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Pontificia Univ Catol Parana, Chem Dept, BR-80215901 Curitiba, PR, BrazilPontificia Univ Catol Parana, Sch Med, Grad Program Hlth Sci, Lab Emerging Infect Dis, BR-80215901 Curitiba, PR, Brazil
Witt, Maria Alice
[2
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Tuon, Felipe Francisco
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Pontificia Univ Catol Parana, Sch Med, Grad Program Hlth Sci, Lab Emerging Infect Dis, BR-80215901 Curitiba, PR, BrazilPontificia Univ Catol Parana, Sch Med, Grad Program Hlth Sci, Lab Emerging Infect Dis, BR-80215901 Curitiba, PR, Brazil
Tuon, Felipe Francisco
[1
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机构:
[1] Pontificia Univ Catol Parana, Sch Med, Grad Program Hlth Sci, Lab Emerging Infect Dis, BR-80215901 Curitiba, PR, Brazil
Introduction: The antibiofilm activity of silver nanoparticles has been extensively investigated in common bacteria. Metallo-beta-lactamase-producing Gram-negative bacteria are hard-to-treat microorganisms with few therapeutic options, and silver nanoparticles were not evaluated on the biofilm of these bacteria. Objectives: The aim of this study was to evaluate the antibiofilm activity of a bone scaffold impregnated with silver nanoparticles in NDM-producing Gram-negative bacilli. Methods: Bone scaffolds from bovine femur were used for the tests and impregnated with silver nanoparticles (50 nm) by physical adsorption. Silver nitrate minimal inhibitory and bactericidal concentrations (MIC and MBC) were performed on NDM-producing Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa. Disc diffusion tests for silver nanoparticles' susceptibility and the quantification of biofilm production on plate and bone with sessile cell count were performed. Results: The MIC results demonstrated that silver nitrate had an antimicrobial effect on all microorganisms, inactivating the growth of isolates from a concentration of 8 mu g/mL. MBC results showed that E. coli 16.211 was the only isolate to present MIC that were different from MBC, with a value of 16 mu g/mL. Conclusion: Bone scaffolds impregnated with silver nanoparticles can significantly reduce the biofilm of multidrug-resistant bacteria. This is a strategical material that can be used as bone implant in different clinical conditions.
机构:
Cent South Univ, Sch Basic Med Sci, Dept Med Microbiol, Changsha 410013, Peoples R ChinaCent South Univ, Sch Basic Med Sci, Dept Med Microbiol, Changsha 410013, Peoples R China
Zhang, Yapeng
Pan, Xuanhe
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Cent South Univ, Sch Basic Med Sci, Dept Med Microbiol, Changsha 410013, Peoples R ChinaCent South Univ, Sch Basic Med Sci, Dept Med Microbiol, Changsha 410013, Peoples R China
Pan, Xuanhe
Liao, Shijing
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First Peoples Hosp Yueyang, Dept Clin Lab, Yueyang 414000, Peoples R ChinaCent South Univ, Sch Basic Med Sci, Dept Med Microbiol, Changsha 410013, Peoples R China
Liao, Shijing
Jiang, Congyuan
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Hunan Anson Biotechnol Co Ltd, Changsha 410008, Peoples R ChinaCent South Univ, Sch Basic Med Sci, Dept Med Microbiol, Changsha 410013, Peoples R China
Jiang, Congyuan
Wang, Linqian
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Cent South Univ, Hunan Canc Hosp, Xiangya Sch Med, Dept Clin Lab,Affiliated Canc Hosp, Changsha 410013, Peoples R ChinaCent South Univ, Sch Basic Med Sci, Dept Med Microbiol, Changsha 410013, Peoples R China
Wang, Linqian
Tang, Yurong
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Cent South Univ, Sch Basic Med Sci, Dept Med Microbiol, Changsha 410013, Peoples R ChinaCent South Univ, Sch Basic Med Sci, Dept Med Microbiol, Changsha 410013, Peoples R China
Tang, Yurong
Wu, Guojun
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Cent South Univ, Sch Basic Med Sci, Dept Med Microbiol, Changsha 410013, Peoples R ChinaCent South Univ, Sch Basic Med Sci, Dept Med Microbiol, Changsha 410013, Peoples R China
Wu, Guojun
Dai, Gan
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Cent South Univ, Sch Basic Med Sci, Dept Med Microbiol, Changsha 410013, Peoples R ChinaCent South Univ, Sch Basic Med Sci, Dept Med Microbiol, Changsha 410013, Peoples R China
Dai, Gan
Chen, Liyu
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Cent South Univ, Sch Basic Med Sci, Dept Med Microbiol, Changsha 410013, Peoples R ChinaCent South Univ, Sch Basic Med Sci, Dept Med Microbiol, Changsha 410013, Peoples R China
机构:
Cent South Univ, Sch Basic Med Sci, Dept Med Microbiol, Changsha 410013, Peoples R ChinaCent South Univ, Sch Basic Med Sci, Dept Med Microbiol, Changsha 410013, Peoples R China
Zhang, Yapeng
Pan, Xuanhe
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Cent South Univ, Sch Basic Med Sci, Dept Med Microbiol, Changsha 410013, Peoples R ChinaCent South Univ, Sch Basic Med Sci, Dept Med Microbiol, Changsha 410013, Peoples R China
Pan, Xuanhe
Liao, Shijing
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First Peoples Hosp Yueyang, Dept Clin Lab, Yueyang 414000, Peoples R ChinaCent South Univ, Sch Basic Med Sci, Dept Med Microbiol, Changsha 410013, Peoples R China
Liao, Shijing
Jiang, Congyuan
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机构:
Hunan Anson Biotechnol Co Ltd, Changsha 410008, Peoples R ChinaCent South Univ, Sch Basic Med Sci, Dept Med Microbiol, Changsha 410013, Peoples R China
Jiang, Congyuan
Wang, Linqian
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Cent South Univ, Hunan Canc Hosp, Xiangya Sch Med, Dept Clin Lab,Affiliated Canc Hosp, Changsha 410013, Peoples R ChinaCent South Univ, Sch Basic Med Sci, Dept Med Microbiol, Changsha 410013, Peoples R China
Wang, Linqian
Tang, Yurong
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Cent South Univ, Sch Basic Med Sci, Dept Med Microbiol, Changsha 410013, Peoples R ChinaCent South Univ, Sch Basic Med Sci, Dept Med Microbiol, Changsha 410013, Peoples R China
Tang, Yurong
Wu, Guojun
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Cent South Univ, Sch Basic Med Sci, Dept Med Microbiol, Changsha 410013, Peoples R ChinaCent South Univ, Sch Basic Med Sci, Dept Med Microbiol, Changsha 410013, Peoples R China
Wu, Guojun
Dai, Gan
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Cent South Univ, Sch Basic Med Sci, Dept Med Microbiol, Changsha 410013, Peoples R ChinaCent South Univ, Sch Basic Med Sci, Dept Med Microbiol, Changsha 410013, Peoples R China
Dai, Gan
Chen, Liyu
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Cent South Univ, Sch Basic Med Sci, Dept Med Microbiol, Changsha 410013, Peoples R ChinaCent South Univ, Sch Basic Med Sci, Dept Med Microbiol, Changsha 410013, Peoples R China