Expression, characterization and antivascular activity of amino acid sequence repeating collagen hexadecapeptide

被引：1

作者：

Yan, Wenjing ^{[1
,2
,3
]}

Huang, Changjin ^{[4
]}

Yan, Yumiao ^{[5
]}

Wang, Pan ^{[1
,2
,3
]}

Yuwen, Weigang ^{[6
]}

Zhu, Chenhui ^{[1
,2
,3
]}

Fu, Rongzhan ^{[1
,2
,3
]}

Duan, Zhiguang ^{[1
,2
,3
]}

Fan, Daidi ^{[1
,2
,3
]}

机构：

[1] Northwest Univ, Engn Res Ctr Western Resource Innovat Med Green Mf, Sch Chem Engn, Minist Educ, Xian 710069, Peoples R China

[2] Northwest Univ, Sch Chem Engn, Shaanxi R&D Ctr Biomaterials& Fermentat Engn, Xian 710069, Peoples R China

[3] Northwest Univ, Biotech & Biomed Res Inst, Xian 710069, Peoples R China

[4] Chinese Acad Med Sci & Peking Union Med Coll, Plast Surg Hosp, Dept Craniomaxillofacial Surg, Beijing, Peoples R China

[5] Xian GaoXin No 1 High Sch, Xian 710119, Peoples R China

[6] Shaanxi Giant Biotechnol Co Ltd, Xian 710065, Shaanxi, Peoples R China

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2024年 / 270卷

关键词：

Recombinant collagen; Sequence repetition; Pichia pastoris; FGF-2; Inhibit angiogenesis; FIBROBLAST-GROWTH-FACTOR; HEPARIN-BINDING; TUMOR ANGIOGENESIS; PEPTIDE; BONE; SKIN; CANCER; OVEREXPRESSION; NORMALIZATION; INHIBITOR;

D O I：

10.1016/j.ijbiomac.2024.131886

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Type V collagen is an essential component of the extracellular matrix (ECM), and its remodeling releases specific protein fragments that can specifically inhibit endothelial cell responses such as proliferation, migration, and invasion. In this study, we have successfully constructed two engineered strains of Pichia pastoris capable of producing recombinant collagen through a new genetic engineering approach. Through high-density fermentation, the expression of 1605 protein and 1610 protein could reach 2.72 g/L and 4.36 g/L. With the increase of repetition times, the yield also increased. Bioactivity analysis showed that recombinant collagen could block the angiogenic effect of FGF-2 on endothelial cells by eliminating FGF-2-induced endothelial cell migration and invasion. Collectively, the recombinant proteins we successfully expressed have a wide range of potential for inhibiting angiogenesis in the biomaterials and biomedical fields.

引用

页数：13

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