Differential Scanning Calorimetry of Proteins and the Two-State Model: Comparison of Two Formulas

被引：0
|
作者：
Yeritsyan, Knarik [1 ]
Badasyan, Artem [1 ]
机构：
[1] Univ Nova Gorica, Mat Res Lab, Vipavska 13, SI-5000 Nova Gorica, Slovenia
来源：
BIOPHYSICA | 2024年 / 4卷 / 02期
关键词：
protein folding; Differential Scanning Calorimetry; heat capacity; two-state model; Hawley model; BARRIER HEIGHTS; DENATURATION; STABILITY; ENERGY; DOMAIN; HEAT; COLD;
D O I：
10.3390/biophysica4020016
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
Differential Scanning Calorimetry (DSC) is a regular and powerful tool to measure the specific heat profile of various materials. In order to connect the measured profile to the properties of a particular protein, a model is required to fit. We discuss here the application of an exact two-state formula with its approximation and process the DSC experimental data on protein folding in water. The approximate formula relies on the smallness of the transition interval, which is different for each protein. With an example of the set of 33 different proteins, we show the practical validity of the approximation and the equivalence of exact and approximate two-state formulas for processing DSC data.
引用
收藏
页码：227 / 237
页数：11
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