Evaluation of the synergistic potential and mechanisms of action for de novo designed cationic antimicrobial peptides

被引：3

作者：

Karapetian, Margarita ^{[1
]}

Alimbarashvili, Evgenia ^{[1
,2
]}

Vishnepolsky, Boris ^{[2
]}

Gabrielian, Andrei ^{[3
]}

Rosenthal, Alex ^{[3
]}

Hurt, Darrell E.

Tartakovsky, Michael ^{[3
]}

Mchedlishvili, Mariam ^{[1
]}

Arsenadze, Davit ^{[1
]}

Pirtskhalava, Malak ^{[2
]}

Zaalishvili, Giorgi ^{[1
,2
]}

机构：

[1] Agr Univ Georgia, Inst Cellular & Mol Biol, Lab Chromatin Biol, 240 David Aghmashenebeli Alley, Tbilisi 0159, Georgia

[2] Ivane Beritashvili Ctr Expt Biomed, Tbilisi 0160, Georgia

[3] NIAID, Off Cyber Infrastruct & Computat Biol, NIH, Bethesda, MD 20892 USA

来源：

HELIYON | 2024年 / 10卷 / 06期

基金：

美国国家科学基金会;

关键词：

CAMP; AMP; Synergy; E; coli; ESCHERICHIA-COLI; MEMBRANE; CELL; ANTIBIOTICS; DAMAGE; MODEL;

D O I：

10.1016/j.heliyon.2024.e27852

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Antimicrobial peptides (AMPs) have emerged as promising candidates in combating antimicrobial resistance - a growing issue in healthcare. However, to develop AMPs into effective therapeutics, a thorough analysis and extensive investigations are essential. In this study, we employed an in silico approach to design cationic AMPs de novo , followed by their experimental testing. The antibacterial potential of de novo designed cationic AMPs, along with their synergistic properties in combination with conventional antibiotics was examined. Furthermore, the effects of bacterial inoculum density and metabolic state on the antibacterial activity of AMPs were evaluated. Finally, the impact of several potent AMPs on E. coli cell envelope and genomic DNA integrity was determined. Collectively, this comprehensive analysis provides insights into the unique characteristics of cationic AMPs.

引用

页数：14

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