Protocol for the derivation of primary cancer stem cell lines from human ependymal tumors

被引：0

作者：

Richman, Cory M. ^{[1
,2
,3
]}

Dirks, Peter B. ^{[1
,2
,4
]}

Taylor, Michael D. ^{[1
,2
,3
,5
,6
,7
]}

Michealraj, Kulandaimanuvel Antony ^{[1
,2
,8
]}

机构：

[1] Hosp Sick Children, Arthur & Sonia Labatt Brain Tumor Res Ctr, Toronto, ON M5G 0A4, Canada

[2] Hosp Sick Children, Dev & Stem Cell Biol Program, Toronto, ON M5G 0A4, Canada

[3] Univ Toronto, Dept Med Biophys, Toronto, ON M5G 1L7, Canada

[4] Hosp Sick Children, Div Neurosurg, Toronto, ON M5G 1X8, Canada

[5] Baylor Coll Med, Dept Pediat Hematol Oncol, Houston, TX 77030 USA

[6] Texas Childrens Hosp, Canc & Hematol Ctr, Houston, TX 77030 USA

[7] Baylor Coll Med, Dan L Duncan Comprehens Canc Ctr, Houston, TX 77030 USA

[8] Univ Pittsburgh, Sch Med, Dept Neurol Surg, Pittsburgh, PA 15213 USA

来源：

STAR PROTOCOLS | 2024年 / 5卷 / 03期

关键词：

Cancer; Cell Biology; Cell Differentiation; Stem Cells;

D O I：

10.1016/j.xpro.2024.103260

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Cancer stem cells (CSCs) established from surgical biopsies closely mimic the human context and can be used to investigate disease mechanisms, genetic fitness, and therapeutic evaluation. Here, we present a protocol for the derivation of primary patient-derived CSC lines from ependymal tumors. We describe the necessary steps, from surgical intervention and biopsy to the dissociation of ependymomas to derive cultures. We then detail procedures for cell line propagation and define the characteristics of these primary cancer cell lines. For complete details on the use and execution of this protocol, please refer to Michealraj et al.1 1

引用

页数：11

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