miR-590-3p Overexpression Improves the Efficacy of hiPSC-CMs for Myocardial Repair

被引：6

作者：

Zhang, Zhiwei ^{[1
]}

Li, Xiaoting ^{[2
,3
]}

Zhuang, Jiawei ^{[4
]}

Ding, Qingwei ^{[1
]}

Zheng, Hui ^{[1
]}

Ma, Teng ^{[1
]}

Meng, Qingyou ^{[1
]}

Gao, Ling ^{[5
,6
]}

机构：

[1] Shanghai Jiao Tong Univ, Shanghai Gen Hosp, Gen Surg Clin Ctr, Dept Vasc Surg,Sch Med, Shanghai, Peoples R China

[2] Shanghai Jiao Tong Univ, Sch Med, Shanghai Gen Hosp, Dept Geriatr, Shanghai, Peoples R China

[3] Soochow Univ, Affiliated Hosp 2, Dept Cardiol, Suzhou, Peoples R China

[4] Xiamen Univ, Sch Med, Affiliated Hosp 1, Dept Cardiovasc Surg, Xiamen, Peoples R China

[5] Tongji Univ, Shanghai East Hosp, Sch Med, Translat Med Ctr Stem Cell Therapy, 1800 Yuntai Rd, Shanghai 200123, Peoples R China

[6] Tongji Univ, Shanghai East Hosp, Inst Regenerat Med, Sch Med, 1800 Yuntai Rd, Shanghai 200123, Peoples R China

来源：

JACC-BASIC TO TRANSLATIONAL SCIENCE | 2024年 / 9卷 / 05期

基金：

中国国家自然科学基金;

关键词：

cell cycle; human induced pluripotent stem cells; miR-590-3p; myocardial infarction; CELL-DERIVED CARDIOMYOCYTES; HEART; CYCLE; MICRORNA; GROWTH; MIRNAS; PKM2;

D O I：

10.1016/j.jacbts.2023.11.005

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Recent evidence demonstrates that low engraftment rates limit the ef ficacy of human induced pluripotent stem cell -derived cardiomyocytes (hiPSC-CMs) for cardiac repair after myocardial infarction. In this study, we attempted to overcome this limitation by enhancing the proliferative capacity of transplanted hiPSC-CMs. We found that miR-590-3p overexpression increased the proliferative capacity of hiPSC-CMs. miR-590-3p overexpression increased the number of engrafted cells and had a higher ef ficacy for myocardial repair than control cells. Moreover, we con firmed the safety of using miR-590-3p-overexpressing hiPSC-CMs in pig hearts. These results indicated that miR-590-3p overexpression stimulated hiPSC-CM cell cycle re-entry to induce cell proliferation and increased the therapeutic ef ficacy in MI. (J Am Coll Cardiol Basic Trans Science 2024;9:557 -573) (c) 2024 The Authors. Published by Elsevier on behalf of the American College of Cardiology Foundation. This is an open access article under the CC BY -NC -ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

引用

页码：557 / 573

页数：17

共 27 条

[1] Reciprocal regulation of miRNAs and piRNAs in embryonic development [J].

Du, W. W. ;

Yang, W. ;

Xuan, J. ;

Gupta, S. ;

Krylov, S. N. ;

Ma, X. ;

Yang, Q. ;

Yang, B. B. .

CELL DEATH AND DIFFERENTIATION, 2016, 23 (09) :1458-1470

[2] Proteasome-Dependent Regulation of Distinct Metabolic States During Long-Term Culture of Human iPSC-Derived Cardiomyocytes [J].

Ebert, Antje ;

Joshi, Amit U. ;

Andorf, Sandra ;

Dai, Yuanyuan ;

Sampathkumar, Shrivatsan ;

Chen, Haodong ;

Li, Yingxin ;

Garg, Priyanka ;

Toischer, Karl ;

Hasenfuss, Gerd ;

Mochly-Rosen, Daria ;

Wu, Joseph C. .

CIRCULATION RESEARCH, 2019, 125 (01) :90-103

[3] Functional screening identifies miRNAs inducing cardiac regeneration [J].

Eulalio, Ana ;

Mano, Miguel ;

Dal Ferro, Matteo ;

Zentilin, Lorena ;

Sinagra, Gianfranco ;

Zacchigna, Serena ;

Giacca, Mauro .

NATURE, 2012, 492 (7429) :376-+

[4] Metabolic Maturation Media Improve Physiological Function of Human iPSC-Derived Cardiomyocytes [J].

Feyen, Dries A. M. ;

McKeithan, Wesley L. ;

Bruyneel, Arne A. N. ;

Spiering, Sean ;

Hoermann, Larissa ;

Ulmer, Barbel ;

Zhang, Hui ;

Briganti, Francesca ;

Schweizer, Michaela ;

Hegyi, Bence ;

Liao, Zhandi ;

Polonen, Risto-Pekka ;

Ginsburg, Kenneth S. ;

Lam, Chi Keung ;

Serrano, Ricardo ;

Wahlquist, Christine ;

Kreymerman, Alexander ;

Vu, Michelle ;

Amatya, Prashila L. ;

Behrens, Charlotta S. ;

Ranjbarvaziri, Sara ;

Maas, Renee G. C. ;

Greenhaw, Matthew ;

Bernstein, Daniel ;

Wu, Joseph C. ;

Bers, Donald M. ;

Eschenhagen, Thomas ;

Metallo, Christian M. ;

Mercola, Mark .

CELL REPORTS, 2020, 32 (03)

[5] MicroRNA therapy stimulates uncontrolled cardiac repair after myocardial infarction in pigs [J].

Gabisonia, Khatia ;

Prosdocimo, Giulia ;

Aquaro, Giovanni Donato ;

Carlucci, Lucia ;

Zentilin, Lorena ;

Secco, Ilaria ;

Ali, Hashim ;

Braga, Luca ;

Gorgodze, Nikoloz ;

Bernini, Fabio ;

Burchielli, Silvia ;

Collesi, Chiara ;

Zandona, Lorenzo ;

Sinagra, Gianfranco ;

Piacenti, Marcello ;

Zacchigna, Serena ;

Bussani, Rossana ;

Recchia, Fabio A. ;

Giacca, Mauro .

NATURE, 2019, 569 (7756) :418-+

[6] Therapeutic role of miR-19a/19b in cardiac regeneration and protection from myocardial infarction [J].

Gao, Feng ;

Kataoka, Masaharu ;

Liu, Ning ;

Liang, Tian ;

Huang, Zhan-Peng ;

Gu, Fei ;

Ding, Jian ;

Liu, Jianming ;

Zhang, Feng ;

Ma, Qing ;

Wang, Yingchao ;

Zhang, Mingming ;

Hu, Xiaoyun ;

Kyselovic, Jan ;

Hu, Xinyang ;

Pu, William T. ;

Wang, Jian'an ;

Chen, Jinghai ;

Wang, Da-Zhi .

NATURE COMMUNICATIONS, 2019, 10 (1)

[7] Exosomes secreted by hiPSC-derived cardiac cells improve recovery from myocardial infarction in swine [J].

Gao, Ling ;

Wang, Lu ;

Wei, Yuhua ;

Krishnamurthy, Prasanna ;

Walcott, Gregory P. ;

Menasche, Philippe ;

Zhang, Jianyi .

SCIENCE TRANSLATIONAL MEDICINE, 2020, 12 (561)

[8] Self-assembling peptide hydrogel enables instant epicardial coating of the heart with mesenchymal stromal cells for the treatment of heart failure [J].

Ichihara, Yuki ;

Kaneko, Masahiro ;

Yamahara, Kenichi ;

Koulouroudias, Marinos ;

Sato, Nobuhiko ;

Uppal, Rakesh ;

Yamazaki, Kenji ;

Saito, Satoshi ;

Suzuki, Ken .

BIOMATERIALS, 2018, 154 :12-23

[9] miR-106a-363 cluster in extracellular vesicles promotes endogenous myocardial repair via Notch3 pathway in ischemic heart injury [J].

Jung, Ji-Hye ;

Ikeda, Gentaro ;

Tada, Yuko ;

von Bornstadt, Daniel ;

Santoso, Michelle R. ;

Wahlquist, Christine ;

Rhee, Siyeon ;

Jeon, Young-Jun ;

Yu, Anthony C. ;

O'brien, Connor G. ;

Red-Horse, Kristy ;

Appel, Eric A. ;

Mercola, Mark ;

Woo, Joseph ;

Yang, Phillip C. .

BASIC RESEARCH IN CARDIOLOGY, 2021, 116 (01)

[10] In Vivo Maturation of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes in Neonatal and Adult Rat Hearts [J].

Kadota, Shin ;

Pabon, Lil ;

Reinecke, Hans ;

Murry, Charles E. .

STEM CELL REPORTS, 2017, 8 (02) :278-289

← 1 2 3 →