Evaluation of Human Platelet Lysate as an Alternative to Fetal Bovine Serum for Potential Clinical Applications of Stem Cells from Human Exfoliated Deciduous Teeth

被引:4
作者
Yoon, Ji-Young [1 ,2 ]
Vu, Huong Thu [2 ,3 ,4 ]
Lee, Jun Hee [1 ,2 ,5 ,6 ,7 ,8 ,9 ,10 ]
Shin, Ji-Sun [4 ]
Kim, Hae-Won [1 ,2 ,5 ,6 ,7 ,8 ,9 ,10 ]
Lee, Hae-Hyoung [2 ,5 ,6 ,7 ,8 ]
Kim, Jong-Bin [4 ]
Lee, Jung-Hwan [1 ,2 ,5 ,6 ,7 ,8 ,9 ,10 ]
机构
[1] Cell & Matter Corp, Inst Stem Cell & Matters, Cheonan 31116, South Korea
[2] Dankook Univ, Inst Tissue Regenerat Engn ITREN, 119 Dandae Ro, Cheonan 31116, South Korea
[3] Univ Med & Pharm Ho Chi Minh City, Fac Odontostomatol, Dept Pediat Dent, Ho Chi Minh City 17000, Vietnam
[4] Dankook Univ, Coll Dent, Dept Pediat Dent, 119 Dandae Ro, Cheonan 31116, South Korea
[5] Dankook Univ, Coll Dent, Dept Biomat Sci, 119 Dandae Ro, Cheonan 31116, South Korea
[6] Dankook Univ, Dept Nanobiomed Sci, 119 Dandae Ro, Cheonan 31116, South Korea
[7] Dankook Univ, FOUR NBM Global Res Ctr Regenerat Med BK21, 119 Dandae Ro, Cheonan 31116, South Korea
[8] Dankook Univ, UCL Eastman Korea Dent Med Innovat Ctr, 119 Dandae Ro, Cheonan 31116, South Korea
[9] Dankook Univ, Mechanobiol Dent Med Res Ctr, 119 Dandae Ro, Cheonan 31116, South Korea
[10] Dankook Univ, Cell & Matter Inst, 119 Dandae Ro, Cheonan 31116, South Korea
关键词
stem cells derived from human exfoliated deciduous teeth; human dental pulp stem cells; human platelet lysate; SHED; DPSC; hPL; cell therapy; FBS alternative; clinical application of stem cells; NEURAL CREST; DENTAL-PULP; STROMAL CELLS; DEATH; TOOTH; STABILITY; EXPANSION; CULTURE; GROWTH; SAFETY;
D O I
10.3390/cells13100847
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
In recent years, there has been a surge in demand for and research focus on cell therapy, driven by the tissue-regenerative and disease-treating potentials of stem cells. Among the candidates, dental pulp stem cells (DPSCs) or human exfoliated deciduous teeth (SHED) have garnered significant attention due to their easy accessibility (non-invasive), multi-lineage differentiation capability (especially neurogenesis), and low immunogenicity. Utilizing these stem cells for clinical purposes requires careful culture techniques such as excluding animal-derived supplements. Human platelet lysate (hPL) has emerged as a safer alternative to fetal bovine serum (FBS) for cell culture. In our study, we assessed the impact of hPL as a growth factor supplement for culture medium, also conducting a characterization of SHED cultured in hPL-supplemented medium (hPL-SHED). The results showed that hPL has effects in enhancing cell proliferation and migration and increasing cell survivability in oxidative stress conditions induced by H2O2. The morphology of hPL-SHED exhibited reduced size and elongation, with a differentiation capacity comparable to or even exceeding that of SHED cultured in a medium supplemented with fetal bovine serum (FBS-SHED). Moreover, no evidence of chromosome abnormalities or tumor formation was detected. In conclusion, hPL-SHED emerges as a promising candidate for cell therapy, exhibiting considerable potential for clinical investigation.
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页数:17
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