Establishing aqueous two-phase flotation coupled with preparative high performance liquid chromatography and its application for the purification of astragalin from Flaveria bidentis

被引：3
作者：
Wei, Tongyu [1 ]
Ni, Hanwen [1 ]
机构：
[1] China Agr Univ, Coll Resources & Environm Sci, Beijing 100193, Peoples R China
来源：
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2024年 / 1240卷
关键词：
Aqueous two-phase flotation; Preparative high performance liquid; chromatography; Astragalin; Flaveria bidentis; SEPARATION; RECOVERY;
D O I：
10.1016/j.jchromb.2024.124141
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The strategy of aqueous two-phase flotation (ATPF) followed by preparative high performance liquid chromatography (prep-HPLC) was established and used for the separation of astragalin from Flaveria bidentis. In the ATPF, the effects of sublation solvent, solution pH, (NH4)2SO4 concentration in aqueous solution, cosolvent, N2 flow rate, flotation time and volumes of the PEG phase on the recovery of astragalin were investigated in detail, and the optimal conditions of ATPF were selected: 50 wt% PEG1000 ethanol solvent as the flotation solvent, pH 4, 350 g/L of (NH4)2SO4 concentration in 5 % ethanol aqueous phase, 40 mL/min of N2 flow rate, 30 min of flotation time, 10.0 mL of flotation solvent volume and twice. After ATPF enrichment, the flotation product was further purified by prep-HPLC. As determined by HPLC, the purity of astragalin was 98.8 %.
引用
收藏
页数：6
相关论文
共 27 条
  • [1] Separation and Purification of Penicillin G from fermentation broth by solvent sublation
    Bi, P. Y.
    Dong, H. R.
    Guo, Q. Z.
    [J]. SEPARATION AND PURIFICATION TECHNOLOGY, 2009, 65 (02) : 228 - 231
  • [2] Separation and concentration of baicalin from Scutellaria Baicalensis Georgi extract by aqueous two-phase flotation
    Bi, Peng-yu
    Chang, Lin
    Mu, Ying-lin
    Liu, Jian-you
    Wu, Yu
    Geng, Xue
    Wei, Yun
    [J]. SEPARATION AND PURIFICATION TECHNOLOGY, 2013, 116 : 454 - 457
  • [3] The recent progress of solvent sublation
    Bi, Peng-yu
    Dong, Hui-ru
    Dong, Jun
    [J]. JOURNAL OF CHROMATOGRAPHY A, 2010, 1217 (16) : 2716 - 2725
  • [4] LEGO MINDSTORMS Fraction Collector: A Low-Cost Tool for a Preparative High-Performance Liquid Chromatography System
    Caputo, Marco
    Lyles, James T.
    Salazar, Monique S.
    Quave, Cassandra L.
    [J]. ANALYTICAL CHEMISTRY, 2020, 92 (02) : 1687 - 1690
  • [5] Extraction of Bioactive Compounds from Haskap Leaves (Lonicera caerulea) Using Salt/Ethanol Aqueous Two-Phase Flotation
    Chong, Kar Yeen
    Stefanova, Roumiana
    Zhang, Junzeng
    Brooks, Marianne Su-Ling
    [J]. FOOD AND BIOPROCESS TECHNOLOGY, 2020, 13 (12) : 2131 - 2144
  • [6] Recovery and concentration of ortho-phenylphenol from biodesulfurization of 4-methyl dibenzothiophene by aqueous two-phase flotation
    de Araujo Padilha, Carlos Eduardo
    Fortunato Dantas, Paulo Victor
    Sousa Junior, Francisco Caninde
    Oliveira Junior, Sergio Dantas
    Nogueira, Cleitiane da Costa
    de Santana Souza, Domingos Fabiano
    de Oliveira, Jackson Araujo
    de Macedo, Gorete Ribeiro
    dos Santos, Everaldo Silvino
    [J]. SEPARATION AND PURIFICATION TECHNOLOGY, 2017, 176 : 306 - 312
  • [7] Determination of pyrethroid pesticide residues in vegetables by solvent sublation followed by high-performance liquid chromatography
    Dong, Huiru
    Bi, Pengyu
    Xi, Yanli
    [J]. JOURNAL OF CHROMATOGRAPHIC SCIENCE, 2008, 46 (07) : 622 - 626
  • [8] Optimization of the subcritical water extraction of sulfated flavonoids from Flaveria bidentis
    Falletti, Paulina
    Barrera Vazquez, Maria Fernanda
    Gonsalves Rodrigues, Luiz Gustavo
    Henrique Santos, Pedro
    Lanza, Marcelo
    Luis Cabrera, Jose
    Evangelina Martini, Raquel
    Raquel Comini, Laura
    [J]. JOURNAL OF SUPERCRITICAL FLUIDS, 2023, 199
  • [9] Development of a continuous aqueous two-phase flotation process for the downstream processing of biotechnological products
    Jakob, Lucas
    Heinzmann, Marcel
    Nirschl, Hermann
    [J]. SEPARATION AND PURIFICATION TECHNOLOGY, 2022, 278
  • [10] Separation and Enrichment of Antioxidant Peptides from Whey Protein Isolate Hydrolysate by Aqueous Two-Phase Extraction and Aqueous Two-Phase Flotation
    Jiang, Bin
    Na, Jiaxin
    Wang, Lele
    Li, Dongmei
    Liu, Chunhong
    Feng, Zhibiao
    [J]. FOODS, 2019, 8 (01):
123