In situ studies of membrane biology by cryo-electron tomography

被引：2
|
作者：
Keller, Jenny [1 ,2 ]
Fernandez-Busnadiego, Ruben [1 ,2 ,3 ,4 ]
机构：
[1] Univ Med Ctr Gottingen, Inst Neuropathol, D-37077 Gottingen, Germany
[2] Univ Gottingen, Collaborat Res Ctr Compartmental Gates & Contact S, Gottingen, Germany
[3] Univ Gottingen, Cluster Excellence Multiscale Bioimaging Mol Machi, D-37077 Gottingen, Germany
[4] Univ Gottingen, Fac Phys, D-37077 Gottingen, Germany
来源：
CURRENT OPINION IN CELL BIOLOGY | 2024年 / 88卷
基金：
欧洲研究理事会;
关键词：
ELECTRON TOMOGRAPHY; CELL; ARCHITECTURE; RESOLUTION;
D O I：
10.1016/j.ceb.2024.102363
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Cryo-electron tomography (cryo-ET) allows high resolution 3D imaging of biological samples in near-native environments. Thus, cryo-ET has become the method of choice to analyze the unperturbed organization of cellular membranes. Here, we briefly discuss current cryo-ET workflows and their application to study membrane biology in situ , under basal and pathological conditions.
引用
收藏
页数：10
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