Myxomatous mitral valve disease and associated pulmonary hypertension might increase serum angiopoietin-2 in dogs

OBJECTIVE To evaluate the relationships between the severity of myxomatous mitral valve disease (MMVD) and pulmonary hypertension (PH) and serum angiopoietin (Ang)-1 and Ang-2 concentrations in dogs with MMVD. ANIMALS 74 dogs (control, n = 12; MMVD, n = 62) were included. METHODS Serum Ang-1 and Ang-2 concentrations were estimated using the canine -specific ELISA kit. The concentrations were compared between dogs with MMVD and healthy dogs, and they were analyzed according to the severity of MMVD and PH. RESULTS The median serum Ang-1 concentration did not differ among the study groups. The median serum Ang-2 con - centration was higher in dogs with stage B2 MMVD ( P = .041) and acute congestive heart failure ( P = .002) than in control dogs. In addition, the median serum Ang-2 concentration was higher in MMVD dogs with PH than in those without PH ( P = .031). Serum Ang-2 concentration was correlated with vertebral heart score ( r s = 0.36, P = .004) and vertebral left atrial score ( r = 0.50, P < .001) in dogs with MMVD, and correlated with vertebral heart score ( r = 0.63, P = .01), maximum E wave amplitude of the diastolic transmitral flow ( r s = 0.61, P = .018), ejection fraction ( r s = -0.77, P < .001) and fractional shortening ( r s = -0.56, P = .032) in dogs with acute congestive heart failure. CLINICAL RELEVANCE Circulating Ang-2 levels increase in dogs with the severity of MMVD and the presence of PH.