A robust ultra-performance liquid chromatography-tandem mass spectrometry method for simultaneous determination of 10 components in glutathione cycle
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作者:
Qin, Xiang-Ling
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Nanjing Univ Chinese Med, Affiliated Hosp Integrated Tradit Chinese & Wester, Dept Pharmaceut Anal, Nanjing 210028, Peoples R ChinaNanjing Univ Chinese Med, Affiliated Hosp Integrated Tradit Chinese & Wester, Dept Pharmaceut Anal, Nanjing 210028, Peoples R China
Qin, Xiang-Ling
[1
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Wang, Si-Yu
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Nanjing Univ Chinese Med, Affiliated Hosp Integrated Tradit Chinese & Wester, Dept Pharmaceut Anal, Nanjing 210028, Peoples R ChinaNanjing Univ Chinese Med, Affiliated Hosp Integrated Tradit Chinese & Wester, Dept Pharmaceut Anal, Nanjing 210028, Peoples R China
Wang, Si-Yu
[1
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Li, Qi-Long
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Nanjing Univ Chinese Med, Affiliated Hosp Integrated Tradit Chinese & Wester, Dept Pharmaceut Anal, Nanjing 210028, Peoples R ChinaNanjing Univ Chinese Med, Affiliated Hosp Integrated Tradit Chinese & Wester, Dept Pharmaceut Anal, Nanjing 210028, Peoples R China
Li, Qi-Long
[1
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Wang, Jun-Jie
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Nanjing Univ Chinese Med, Affiliated Hosp Integrated Tradit Chinese & Wester, Dept Pharmaceut Anal, Nanjing 210028, Peoples R ChinaNanjing Univ Chinese Med, Affiliated Hosp Integrated Tradit Chinese & Wester, Dept Pharmaceut Anal, Nanjing 210028, Peoples R China
Wang, Jun-Jie
[1
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Yao, Zhong-Wei
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Nanjing Univ Chinese Med, Affiliated Hosp Integrated Tradit Chinese & Wester, Dept Pharmaceut Anal, Nanjing 210028, Peoples R ChinaNanjing Univ Chinese Med, Affiliated Hosp Integrated Tradit Chinese & Wester, Dept Pharmaceut Anal, Nanjing 210028, Peoples R China
Yao, Zhong-Wei
[1
]
Zhu, Jin-Hao
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Nanjing Univ Chinese Med, Affiliated Hosp Integrated Tradit Chinese & Wester, Dept Pharmaceut Anal, Nanjing 210028, Peoples R ChinaNanjing Univ Chinese Med, Affiliated Hosp Integrated Tradit Chinese & Wester, Dept Pharmaceut Anal, Nanjing 210028, Peoples R China
Zhu, Jin-Hao
[1
]
Chen, Lin-Xia
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Nanjing Univ Chinese Med, Affiliated Hosp Integrated Tradit Chinese & Wester, Dept Pharmaceut Anal, Nanjing 210028, Peoples R ChinaNanjing Univ Chinese Med, Affiliated Hosp Integrated Tradit Chinese & Wester, Dept Pharmaceut Anal, Nanjing 210028, Peoples R China
Chen, Lin-Xia
[1
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Huo, Jie-Ge
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Nanjing Univ Chinese Med, Affiliated Hosp Integrated Tradit Chinese & Wester, Dept Pharmaceut Anal, Nanjing 210028, Peoples R ChinaNanjing Univ Chinese Med, Affiliated Hosp Integrated Tradit Chinese & Wester, Dept Pharmaceut Anal, Nanjing 210028, Peoples R China
Huo, Jie-Ge
[1
]
Li, Song-Lin
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Nanjing Univ Chinese Med, Affiliated Hosp Integrated Tradit Chinese & Wester, Dept Pharmaceut Anal, Nanjing 210028, Peoples R ChinaNanjing Univ Chinese Med, Affiliated Hosp Integrated Tradit Chinese & Wester, Dept Pharmaceut Anal, Nanjing 210028, Peoples R China
Li, Song-Lin
[1
]
Zhou, Jing
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Nanjing Univ Chinese Med, Affiliated Hosp Integrated Tradit Chinese & Wester, Dept Pharmaceut Anal, Nanjing 210028, Peoples R ChinaNanjing Univ Chinese Med, Affiliated Hosp Integrated Tradit Chinese & Wester, Dept Pharmaceut Anal, Nanjing 210028, Peoples R China
Zhou, Jing
[1
]
Zhu, He
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Nanjing Med Univ, Affiliated Taizhou Peoples Hosp, Drug Clin Trial Ctr, Taizhou, Peoples R China
Nanjing Univ Chinese Med, Affiliated Hosp Integrated Tradit Chinese & Wester, Nanjing, Peoples R ChinaNanjing Univ Chinese Med, Affiliated Hosp Integrated Tradit Chinese & Wester, Dept Pharmaceut Anal, Nanjing 210028, Peoples R China
Zhu, He
[2
,3
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机构:
[1] Nanjing Univ Chinese Med, Affiliated Hosp Integrated Tradit Chinese & Wester, Dept Pharmaceut Anal, Nanjing 210028, Peoples R China
[2] Nanjing Med Univ, Affiliated Taizhou Peoples Hosp, Drug Clin Trial Ctr, Taizhou, Peoples R China
[3] Nanjing Univ Chinese Med, Affiliated Hosp Integrated Tradit Chinese & Wester, Nanjing, Peoples R China
Glutathione (GSH) is an important antioxidant that is generated and degraded via the GSH cycle. Quantification of the main components in the GSH cycle is necessary to evaluate the process of GSH. In this study, a robust ultra-performance liquid chromatography-tandem mass spectrometry method for the simultaneous quantification of 10 components (GSH; gamma-glutamylcysteine; cysteinyl-glycine; n-acetylcysteine; homocysteine; cysteine; cystine; methionine; glutamate; pyroglutamic acid) in GSH cycle was developed. The approach was optimized in terms of derivative, chromatographic, and spectrometric conditions as well as sample preparation. The unstable thiol groups of GSH, gamma-glutamylcysteine, cysteinyl-glycine, n-acetylcysteine, cysteine, and homocysteine were derivatized by n-ethylmaleimide. The derivatized and underivatized analytes were separated on an amino column with gradient elution. The method was further validated in terms of selectivity (no interference), linearity (R-2 > 0.99), precision (% relative standard deviation [RSD%] range from 0.57 to 10.33), accuracy (% relative error [RE%] range from -3.42 to 10.92), stability (RSD% < 5.68, RE% range from -2.54 to 4.40), recovery (RSD% range from 1.87 to 7.87) and matrix effect (RSD% < 5.42). The validated method was applied to compare the components in the GSH cycle between normal and oxidative stress cells, which would be helpful in clarifying the effect of oxidative stress on the GSH cycle.
机构:
Univ Michigan, Div Gastroenterol & Hepatol, Ann Arbor, MI 48109 USA
VA Ann Arbor Healthcare Syst, Gastroenterol Sect, Ann Arbor, MI USAUniv Edinburgh, Ctr Regenerat Med, 5 Little France Dr, Edinburgh EH16 4UU, Midlothian, Scotland
机构:
Univ Kentucky, Dept Chem, Lexington, KY USA
Univ Kentucky, Sanders Brown Ctr Aging, Lexington, KY 40536 USAUniv Kentucky, Dept Chem, Lexington, KY USA
机构:
Weifang Med Univ, Affiliated Hosp, Dept Pharm, Weifang, Peoples R ChinaWeifang Med Univ, Affiliated Hosp, Dept Pharm, Weifang, Peoples R China
Ding, Chuan-Hua
Zhu, He
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Nanjing Univ Chinese Med, Affiliated Hosp Integrated Tradit Chinese & Weste, Nanjing, Peoples R China
Jiangsu Prov Acad Tradit Chinese Med, Dept Pharmaceut Anal & Metab, Nanjing, Peoples R ChinaWeifang Med Univ, Affiliated Hosp, Dept Pharm, Weifang, Peoples R China
机构:
Univ Calif Merced, Merced, CA 95343 USA
Univ Southern Calif, Leonard Davis Sch Gerontol, Los Angeles, CA 90007 USAUniv Calif Merced, Merced, CA 95343 USA
Forman, Henry Jay
Zhang, Hongqiao
论文数: 0引用数: 0
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Univ Southern Calif, Leonard Davis Sch Gerontol, Los Angeles, CA 90007 USAUniv Calif Merced, Merced, CA 95343 USA
机构:
Univ Michigan, Div Gastroenterol & Hepatol, Ann Arbor, MI 48109 USA
VA Ann Arbor Healthcare Syst, Gastroenterol Sect, Ann Arbor, MI USAUniv Edinburgh, Ctr Regenerat Med, 5 Little France Dr, Edinburgh EH16 4UU, Midlothian, Scotland
机构:
Univ Kentucky, Dept Chem, Lexington, KY USA
Univ Kentucky, Sanders Brown Ctr Aging, Lexington, KY 40536 USAUniv Kentucky, Dept Chem, Lexington, KY USA
机构:
Weifang Med Univ, Affiliated Hosp, Dept Pharm, Weifang, Peoples R ChinaWeifang Med Univ, Affiliated Hosp, Dept Pharm, Weifang, Peoples R China
Ding, Chuan-Hua
Zhu, He
论文数: 0引用数: 0
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机构:
Nanjing Univ Chinese Med, Affiliated Hosp Integrated Tradit Chinese & Weste, Nanjing, Peoples R China
Jiangsu Prov Acad Tradit Chinese Med, Dept Pharmaceut Anal & Metab, Nanjing, Peoples R ChinaWeifang Med Univ, Affiliated Hosp, Dept Pharm, Weifang, Peoples R China
机构:
Univ Calif Merced, Merced, CA 95343 USA
Univ Southern Calif, Leonard Davis Sch Gerontol, Los Angeles, CA 90007 USAUniv Calif Merced, Merced, CA 95343 USA
Forman, Henry Jay
Zhang, Hongqiao
论文数: 0引用数: 0
h-index: 0
机构:
Univ Southern Calif, Leonard Davis Sch Gerontol, Los Angeles, CA 90007 USAUniv Calif Merced, Merced, CA 95343 USA