基于rDNA-ITS2序列的中国按蚊属塞蚊亚属部分种类的系统发育研究(双翅目:蚊科)

被引:11
作者
马雅军
吴静
马颖
机构
[1] 第二军医大学病原生物学教研室
关键词
双翅目; 按蚊属; 塞蚊亚属; rDNA-ITS2; 系统发育;
D O I
暂无
中图分类号
Q969 [昆虫分类学];
学科分类号
07 ; 0710 ; 09 ;
摘要
应用rDNA-ITS2序列,采用邻接法(NJ)、最大简约法(MP)和最大似然法(ML),以按蚊亚属Anopheles的中华按蚊An.(An.)sinensis和赫坎按蚊An.(An.)hyrcanus为外群,对采自中国的按蚊属Anopheles塞蚊亚属Cellia21种蚊进行了系统发育分析。结果表明:rDNA-ITS2序列的长度范围为435~838bp,塞蚊亚属蚊种间遗传距离为0.027~0.496,平均值为0.336。各种方法重建的系统发育树均显示新迈蚊系Neomyzomyia为单系,新塞蚊系Neocellia和迈蚊系Myzomyia为并系。21种蚊在系统发育树中均分为4支,伪威氏按蚊An.pseudowillmori和塞沃按蚊An.sawadwongporni未归入多斑按蚊种团(An.maculatus group),其它各种团和复合体成员种的聚类关系与形态分类结果一致。本研究重建的分子系统发育树阐明了中国按蚊属塞蚊亚属各系及蚊种之间的亲缘关系,ITS2序列具有足够的信息贡献量。
引用
收藏
页码:245 / 256
页数:12
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