白血病骨髓微环境中GATA-1、GATA-2和GATA-3基因表达模式(英文)

Objective: The aim of the study was to investigate the expression pattern of hematopoietic transcription factor GATA-1, -2 and -3 genes in leukemic bone marrow (BM) microenvironment [including bone marrow stromal cells (BMSCs) and BM hematopoietic cells]. Methods: Mononuclear cells were isolated from BM of patients with acute myeloid leukemia (AML), chronic myelogenous leukemia (CML), or acute lymphoblastic leukemia (ALL). Adherent cells (BMSCs) and nonadherent cells (BM hematopoietic cells) were collected after long-term culture in vitro. The semi-quantitative expression levels of GATA genes in the BMSCs or BM hematopoietic cells from patients with leukemia were analyzed by using RT-PCR-ELISA and compared with normal controls. Results: The expression level of GATA-1 gene in the BMSCs from CML group was significantly lower than that of the normal controls. The expression level of GATA-3 gene in the BMSCs from ALL was higher than that of the normal controls, but that from CML was lower than the normal controls. Dominant expression of GATA-3 gene was found in the normal BM hematopoietic cells. The dominant expression of GATA-2 gene was found in the normal BMSCs and the BMSCs from CML, whereas the dominant expression of GATA-3 gene was detected in the BMSCs from AML. Conclusion: GATA-1, -2 and -3 genes might play a role in hematopoiesis regulation in leukemia, and the changes of expression pattern of GATA genes might influence the hematopoiesis in BM microenvironment and relate to the pathogenesis and development of leukemia.