Cloning and functional analysis of chloroplast division gene NtFtsZ2-l in Nicotiana tabacum

被引:3
作者
KONG Dongdong
2. Department of Biology
3. Laboratory of Agronomy and Biotechnology
4. College of Life Sciences
机构
基金
中国国家自然科学基金;
关键词
Nicotiana tabacum; NtFtsZ2-l gene; chloroplast division;
D O I
暂无
中图分类号
Q785 [转化及克隆];
学科分类号
071007 ; 0836 ; 090102 ;
摘要
FtsZ protein plays an important role in the division of chloroplasts. With the finding and functional analysis of higher plant FtsZ proteins, people have deepened the understanding in the molecular mechanism of chloroplast division. Multiple ftsZ genes are diversified into two families in higher plants, ftsZ1 and ftsZ2 . On the basis of the research on ftsZl family, we analyzed the function of NtFtsZ2-l gene in Nicotiana tabacum . Microscopic analysis of the sense and antisense NtFtsZ2-l transgenic tobacco plants revealed that the chloroplasts were abnormal in size and also in number when compared with wild-type tobacco chloroplasts. Our investigations confirmed that the NtFtsZ2-l gene is involved in plant chloroplast division.
引用
收藏
页码:39 / 43
页数:5
相关论文
共 8 条
  • [1] Cloning of two plastid division ftsZ genes from Nicotiana tabacum and their expression in E. coli[J]. WANG Dong KONG Dongdong HU Yong JU Chuanli HE Yikun and SUN Jingsan(1. Department of Biology, Capital Normal University, Beijing 100037, China;2. Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China;3. College of Life Sciences, Beijing Normal University, Beijing 100875, China).Progress in Natural Science. 2002(12)
  • [2] Cloning of plastid division gene GlFtsZ from Gentiana lutea and its expression during petal development[J]. HE Yikun , ZHU Changfu , WANG Dong, KONG Dongdong and SUN Jingsan(1. Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China;2. Department of Biology, Capital Normal University, Beijing 100037, China;3. College of Life Science, Northeast Normal University, Changchun 130024, China).Progress in Natural Science. 2002(08)
  • [3] 烟草质体分裂相关基因NtFtsZ1和NtFtsZ2对叶绿体分裂和形态的影响(英文)
    王东
    孔冬冬
    鞠传丽
    胡勇
    何奕昆
    孙敬三
    [J]. ActaBotanicaSinica, 2002, (07) : 838 - 844
  • [4] Isolation of theftsZ gene from plastid-deficient generative cells ofLilium longiflorum
    Toshiyuki Mori
    Ichiro Tanaka
    [J]. Protoplasma, 2000, 214 : 57 - 64
  • [5] Pea chloroplast FtsZ can form multimers and correct the thermosensitive defect of an Escherichia coli ftsZ mutant
    Gaikwad, A
    Babbarwal, V
    Pant, V
    Mukherjee, SK
    [J]. MOLECULAR AND GENERAL GENETICS, 2000, 263 (02): : 213 - 221
  • [6] The chloroplast division protein FtsZ is encoded by a nucleomorph gene in cryptomonads[J] . M. J. Fraunholz,E. Moerschel,U.-G. Maier.MGG - Molecular and General Genetics . 1998 (2-3)
  • [7] Trends
    [J]. CEW, Chemical Engineering World, 1998, 33 (06):
  • [8] A plant DNA minipreparation: Version II[J] . Stephen L. Dellaporta,Jonathan Wood,James B. Hicks.Plant Molecular Biology Reporter . 1983 (4)