Construction of a genetic map with SRAP markers and localization of the gene responsible for the first-flower-node trait in cucumber (Cucumis sativus L.)

被引:0
作者
PAN Junsong WANG Gang LI Xiaozun HE Huanle WU Aizhong and CAI RunAgriculture and Biology School Shanghai Jiaotong University Shanghai China [201101 ]
机构
关键词
cucumber (Cucumis sativus L.); SRAP (sequence-related amplified polymorphism); linkage map; first flower node;
D O I
暂无
中图分类号
S642.2 [黄瓜];
学科分类号
090202 ;
摘要
With an F 2 population from the cross of two cucumber inbred lines, S06 and S52, sequence-related amplified polymorphism (SRAP) was used to construct a genetic linkage map in cucumber (Cucumis sativus L.). Sixty-four SRAP primer combinations generated 108 polymorphic bands in the F 2 population analysis. The average of polymorphic bands produced by one primer pair was 1.5, and the maximum was 5. Using Mapmaker 3.0, a linkage map was constructed, which consisted of 77 SRAP markers distributed in nine linkage groups (LOD≥3.0) and spanned 1114.2 cM with an average interval of 14.5 cM between markers. The gene for the first-flower-node trait, termed ffn, was mapped to linkage group Ⅸ, flanked by DC1EM5 and ME7EM2A at 10.3 cM and 12.1 cM distance, respectively.
引用
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页码:407 / 413
页数:7
相关论文
共 8 条
[1]  
Construction of a genetic linkage map for cotton based on SRAP[J] . Zhongxu Lin,Xianlong Zhang,Yichun Nie,Daohua He,Maoqing Wu.Chinese Science Bulletin . 2003 (19)
[2]   Gene for gene alignment between the Brassica and Arabidopsis genomes by direct transcriptome mapping [J].
Li, G ;
Gao, M ;
Yang, B ;
Quiros, CF .
THEORETICAL AND APPLIED GENETICS, 2003, 107 (01) :168-180
[3]  
Genetic mapping and QTL analysis of horticultural traits in cucumber ( Cucumis sativus L.) using recombinant inbred lines[J] . G. Fazio,J. E. Staub,M. R. Stevens.Theoretical and Applied Genetics . 2003 (5)
[4]   Genetic diversity of a germplasm collection of Cucurbita pepo using SRAP and AFLP markers [J].
Ferriol, M ;
Picó, B ;
Nuez, F .
THEORETICAL AND APPLIED GENETICS, 2003, 107 (02) :271-282
[5]  
Sequence-related amplified polymorphism (SRAP), a new marker system based on a simple PCR reaction: its application to mapping and gene tagging in Brassica[J] . G. Li,C. F. Quiros.TAG Theoretical and Applied Genetics . 2001 (2-3)
[6]  
Mapping and QTL analysis of horticultural traits in a narrow cross in cucumber ( Cucumis sativus L.) using random-amplified polymorphic DNA markers[J] . Felix C. Serquen,J Bacher,JE Staub.Molecular Breeding . 1997 (4)
[7]   RFLP variation and genetic relationships in cultivated cucumber [J].
Dijkhuizen, A ;
Kennard, WC ;
Havey, MJ ;
Staub, JE .
EUPHYTICA, 1996, 90 (01) :79-87
[8]  
Linkages among RFLP, RAPD, isozyme, disease-resistance, and morphological markers in narrow and wide crosses of cucumber[J] . A. Dijkhuizen,V. Meglic,J. E. Staub,M. J. Havey.Theoretical and Applied Genetics . 1994 (1)