Studies on Immobilization of Aminoacylase From Aspergillus Oryzae on Macroporous Carriers

<正> Synthesized macroporous cross-linked copolymers of methyl acrylate-divinyl benzene (MA-DVB), acrylarnide-N,N'-methylenebisacrylamide (AAM-BIS) and their functionalized products were used for immobilization of aminoacylase from Aspergillus oryzae. Effects of the carrier properties on the activity of immobilized aminoacylase were investigated and effects of substrate concentration, pH, phosphate buffer concentration and temperature on the immobiIized aminoacylase were compared with those of the soluble aminoacylase. A cohmn of immobilized aminoacylase was prepared and used for continuous resolution of N-acyl-DL-methionine; the operational stability of iramobilized enzyme was also investigated.