Microdissection and Microcloning of Human Chromosome and Chromosome Band-specific Painting

被引：0

作者：

邓汉湘

何小轩

戴和平

李麓芸

夏家辉

机构：

[1] Changsha 410078

[2] Hunan Medical University

[3] PRC

[4] State Key Laboratory of Medical Genetics

来源：

ProgressinNaturalScience | 1994年 / 01期

关键词：

microdissection; microcloning; chromosome painting; human chromosome;

D O I：

暂无

中图分类号：

R394 [医学遗传学];

学科分类号：

0710 ; 071007 ;

摘要：

Chromosome painting is a method of fluorescence in situ suppression hybridization using a chromosome-specific DNA library as a probe pool that depicts an entire chromosome or a chromosomal region. We developed a simple method with which a defined chromosome region or band is painted. It involves microdissection of a chromosome, chromosomal region or band; polymerase chain reaction(PCR)amplification of chromosomal DNAs; biotin-labeling of the PCR product; CISS hybridization; and detection of the hybridization signal with FITC-conjugated avidin. With this method, we

引用

页码：119 / 123

页数：5