Effects of theabrownin on brain metabolites in d-galactose-induced aging in mice

被引：0
|
作者：
Lei S. [1 ]
Xie G. [2 ]
Zhang Z. [1 ]
Miao Y. [1 ]
Zhao C. [1 ]
Chen D. [3 ]
Hou Y. [1 ,2 ]
Gong J. [1 ]
机构：
[1] College of Food Science and Technology, Yunnan Agricultural University, Kunming
[2] College of Tea, Yunnan Agricultural University, Kunming
[3] College of Veterinary Medicine, Yunnan Agricultural University, Kunming
来源：
Science and Technology of Food Industry | 2023年 / 44卷 / 05期
关键词：
Anti-aging; Brain metabonomics; Immunocytochemistry; Theabrownin;
D O I：
10.13386/j.issn1002-0306.2022050204
中图分类号：
学科分类号：
摘要：
The objective of the study is to investigate the effect of theabrownin on the immunohistochemistry of brain tissue in aging mice, screen the differential metabolites and analyze the metabolic pathways involved. A mouse aging model was established by subcutaneous injection of D-galactose in mice. Immunohistochemical and UHPLC-QE-MS metabolomics techniques were used to detect brain samples of normal control mice, D-galactose model mice and theabrownin-treated (pretreatment and treatment) mice. The results showed that the areal density of choline acetyltransferase positive expression was significantly increased (P<0.05) after theabrownin intervention. Prophylactic administration of theabrownin could significantly regulate 17 metabolites in brain tissue of aging mice, mainly involving glycerophospholipid metabolism, histidine metabolism, TCA cycle, glyoxylate and dicarboxylate metabolism and beta-alanine metabolism. Theabrownin treatment could significantly regulate 32 metabolites in brain tissue of aging mice, mainly involving glycerophospholipid metabolism, histidine metabolism, taurine and hypotaurine metabolism, valine, leucine and isoleucine degradation, phenylalanine, tyrosine and tryptophan biosynthesis. Carnosine, LysoPC(P-18:1(9Z)), PS(20:5(5Z,8Z,11Z,14Z,17Z)/18:1(9Z)), PC(15:0/15:0), D-arabinose 5-phosphate, cholesterol sulfate, adenosine diphosphate ribose, glycerophospholipid metabolism and histidine metabolism were common in the theabrownin pretreatment and theabrownin treatment groups. Theabrownin can play an anti-aging role by regulating lipid metabolism, energy metabolism, amino acid metabolism and other processes in mice. © 2023, Editorial Department of Science and Technology of Food Science. All rights reserved.
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页码：43 / 52
页数：9
相关论文
共 33 条
  • [1] LIANG Y, ZHANG L, LU J., A study on chemical estimation of Pu-erh tea quality[J], Journal of the Science of Food & Agriculture, 85, 3, pp. 381-390, (2005)
  • [2] ZHAO Z J, TONG H R, ZHOU L, Et al., Fungal colonization of Pu-erh tea in Yunnan[J], Journal of Food Safety, 30, 4, pp. 769-784, (2010)
  • [3] QIN Y, GONG J S, ZHANG H F, Et al., Extraction technology of theabrownine from Pu-erh tea and its physico-chemical properties[J], Chemistry & Industry of Forest Products, 29, 5, pp. 95-98, (2009)
  • [4] MARGALEF M, IGLESISS-CARRES L, PONS Z, Et al., Age related differences in the plasma kinetics of flavanols in rats[J], Journal of Nutritional Biochemistry, 29, pp. 90-96, (2016)
  • [5] JADOON S, KARIM S, ASAD M H H B, Et al., Anti-aging potential of phytoextract loaded-pharmaceutical creams for human skin cell longetivity[J], Oxida Med Cell Longe, 1, (2015)
  • [6] LEI S W, ZHANG Z F, XIE G H, Et al., Theabrownin modulates the gut microbiome and serum metabolome in aging mice induced by D-galactose[J], Journal of Functional Foods, 89, (2022)
  • [7] ZHOU M L, YU Y F, LUO X X, Et al., Study on potential biomarkers and metabolic pathways of rats in different progressive stages of coronary heart disease with blood stasis syndrome based on metabonomics technology[J], Chinese Journal of Information on TCM, 29, 1, pp. 73-79, (2022)
  • [8] YUE S J, LIU J, GONG J S., Effects of theabrownin extracted from Pu-erh tea on the intestinal flora[J], Journal of Tea Science, 36, 3, pp. 261-267, (2016)
  • [9] WANG L Y, YANG L, ZHU J H., Effects of Kangfuxin on learning and memory ability and basal forebrain cholinergic neurons of ovariectomized rats[J], Anatomy Research, 43, 5, pp. 479-483, (2021)
  • [10] CHEN Q L, CHEN Q, JIN B B., The influence of tenuigenin on learning-memory and the activity of marker enzymes of central cholinergic system in AD mice[J], Chinese Remedies & Clinics, 27, 3, pp. 33-36, (2011)
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