Protective effect of ghrelin in oxidative stress-induced age-related macular degeneration in vitro and in vivo

被引：1

作者：

Bai, Jie ^{[1
,2
]}

Wang, Yanqing ^{[1
,2
]}

Li, Yanze ^{[1
,2
]}

Liu, Yan ^{[3
]}

Wang, Shan ^{[4
,5
]}

机构：

[1] Zhejiang Univ, Affiliated Hosp 4, Sch Med, Dept Ophthalmol, Yiwu 322000, Zhejiang, Peoples R China

[2] Zhejiang Univ, Int Inst Med, Int Sch Med, Yiwu 322000, Zhejiang, Peoples R China

[3] Yiwu Second Peoples Hosp, Dept Ophthalmol & Otorhinolaryngol, Yiwu 322000, Zhejiang, Peoples R China

[4] Hainan Med Univ, Sch Stomatol, Dept Oral Pathol, Haikou 571199, Peoples R China

[5] Hainan Med Coll, Sch Stomatol, Dept Oral Pathol, Haikou 571199, Peoples R China

来源：

MOLECULAR MEDICINE | 2024年 / 30卷 / 01期

关键词：

Ghrelin; Oxidative stress; Age-related macular degeneration; Vitro and vivo; APOPTOSIS; CELLS;

D O I：

10.1186/s10020-024-00920-w

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Oxidative damage to human retinal pigment epithelial (RPE) cells is the main cause of age-related macular degeneration (AMD), in our previous work, we showed that ghrelin has an antioxidative effect on human lens epithelium (HLE) cells, however, the studies of using ghrelin in treating the degenerative diseases of the retina have rarely been reported. In this article, we assessed the effect of ghrelin on preventing oxidative stress induced by hydrogen peroxide (H2O2) in ARPE-19 cells and its mechanism. We observed that pretreatment with ghrelin protected ARPE-19 cells from H2O2-induced cell oxidative injuries and apoptosis responses. Furthermore, an oxidative stress-induced mouse model of AMD was established via injection of sodium iodate (NaIO3) to tail veins, and treatment with ghrelin preserved retinal function, and protected photoreceptors. The first to report ghrelin' protective effect on H2O2-induced RPE cells.Treatment with ghrelin inhibits NaIO3-induced retinal cell apoptosis in vivo.Combined with in vitro and in vivo experiments, the results are comprehensive and reliable.

引用

页数：10

共 28 条

[11] Landmark trials in ophthalmology: The age-related eye diseases studies [J].