Protective effect of ghrelin in oxidative stress-induced age-related macular degeneration in vitro and in vivo

被引:0
作者
Bai, Jie [1 ,2 ]
Wang, Yanqing [1 ,2 ]
Li, Yanze [1 ,2 ]
Liu, Yan [3 ]
Wang, Shan [4 ,5 ]
机构
[1] Zhejiang Univ, Affiliated Hosp 4, Sch Med, Dept Ophthalmol, Yiwu 322000, Zhejiang, Peoples R China
[2] Zhejiang Univ, Int Inst Med, Int Sch Med, Yiwu 322000, Zhejiang, Peoples R China
[3] Yiwu Second Peoples Hosp, Dept Ophthalmol & Otorhinolaryngol, Yiwu 322000, Zhejiang, Peoples R China
[4] Hainan Med Univ, Sch Stomatol, Dept Oral Pathol, Haikou 571199, Peoples R China
[5] Hainan Med Coll, Sch Stomatol, Dept Oral Pathol, Haikou 571199, Peoples R China
关键词
Ghrelin; Oxidative stress; Age-related macular degeneration; Vitro and vivo; APOPTOSIS; CELLS;
D O I
10.1186/s10020-024-00920-w
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Oxidative damage to human retinal pigment epithelial (RPE) cells is the main cause of age-related macular degeneration (AMD), in our previous work, we showed that ghrelin has an antioxidative effect on human lens epithelium (HLE) cells, however, the studies of using ghrelin in treating the degenerative diseases of the retina have rarely been reported. In this article, we assessed the effect of ghrelin on preventing oxidative stress induced by hydrogen peroxide (H2O2) in ARPE-19 cells and its mechanism. We observed that pretreatment with ghrelin protected ARPE-19 cells from H2O2-induced cell oxidative injuries and apoptosis responses. Furthermore, an oxidative stress-induced mouse model of AMD was established via injection of sodium iodate (NaIO3) to tail veins, and treatment with ghrelin preserved retinal function, and protected photoreceptors. The first to report ghrelin' protective effect on H2O2-induced RPE cells.Treatment with ghrelin inhibits NaIO3-induced retinal cell apoptosis in vivo.Combined with in vitro and in vivo experiments, the results are comprehensive and reliable.
引用
收藏
页数:10
相关论文
共 28 条
[1]   Ghrelin Ameliorates Diabetic Retinal Injury: Potential Therapeutic Avenues for Diabetic Retinopathy [J].
Bai, Jie ;
Yang, Fan ;
Wang, Ruiqi ;
Yan, Qinghui .
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY, 2021, 2021
[2]   Ghrelin Protects Human Lens Epithelial Cells against Oxidative Stress-Induced Damage [J].
Bai, Jie ;
Yang, Fan ;
Dong, Li ;
Zheng, Yi .
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY, 2017, 2017
[3]   Neuroprotective and antioxidant effects of ghrelin in an experimental glaucoma model [J].
Can, Nagehan ;
Catak, Onur ;
Turgut, Burak ;
Demir, Tamer ;
Ilhan, Nevin ;
Kuloglu, Tuncay ;
Ozercan, Ibrahim Hanifi .
DRUG DESIGN DEVELOPMENT AND THERAPY, 2015, 9 :2819-2829
[4]   The interplay of autophagy and oxidative stress in the pathogenesis and therapy of retinal degenerative diseases [J].
Chang, Kun-Che ;
Liu, Pei-Feng ;
Chang, Chia-Hsuan ;
Lin, Ying-Cheng ;
Chen, Yen-Ju ;
Shu, Chih-Wen .
CELL AND BIOSCIENCE, 2022, 12 (01)
[5]   Oxidative stress differentially impacts apical and basolateral secretion of angiogenic factors from human iPSC-derived retinal pigment epithelium cells [J].
Chen, Lisheng ;
Perera, N. Dayanthi ;
Karoukis, Athanasios J. ;
Feathers, Kecia L. ;
Ali, Robin R. ;
Thompson, Debra A. ;
Fahim, Abigail T. .
SCIENTIFIC REPORTS, 2022, 12 (01)
[6]   Pretreatment of ghrelin protects H9c2 cells against hypoxia/reoxygenation-induced cell death via PI3K/AKT and AMPK pathways [J].
Chen, Yanbo ;
Wang, Honggang ;
Zhang, Yong ;
Wang, Zhengping ;
Liu, Shanshan ;
Cui, Lianqun .
ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY, 2019, 47 (01) :2179-2187
[7]  
Davies JS, 2022, VITAM HORM, V118, P337, DOI 10.1016/bs.vh.2021.12.003
[8]   Age-related macular degeneration: Epidemiology, genetics, pathophysiology, diagnosis, and targeted therapy [J].
Deng, Yanhui ;
Qiao, Lifeng ;
Du, Mingyan ;
Qu, Chao ;
Wan, Ling ;
Li, Jie ;
Huang, Lulin .
GENES & DISEASES, 2022, 9 (01) :62-79
[9]   Anti-VEGF Monotherapy Versus Photodynamic Therapy and Anti-VEGF Combination Treatment for Neovascular Age-Related Macular Degeneration: A Meta-Analysis [J].
Gao, Yang ;
Yu, Tao ;
Zhang, Yue ;
Dang, Guangfu .
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2018, 59 (10) :4307-4317
[10]   Hypoxia aggravates ferroptosis in RPE cells by promoting the Fenton reaction [J].
Henning, Yoshiyuki ;
Blind, Ursula Sarah ;
Larafa, Safa ;
Matschke, Johann ;
Fandrey, Joachim .
CELL DEATH & DISEASE, 2022, 13 (07)