In Vivo Evaluation of Innovative Gadolinium-Based Contrast Agents Designed for Bioimaging Applications

被引：1

作者：

Voicu, Sorina Nicoleta ^{[1
]}

Gheran, Cecilia Virginia ^{[1
]}

Balta, Cornel ^{[2
]}

Hermenean, Anca ^{[2
]}

Callewaert, Maite ^{[3
]}

Chuburu, Francoise ^{[3
]}

Dinischiotu, Anca ^{[1
]}

机构：

[1] Univ Bucharest, Fac Biol, Dept Biochem & Mol Biol, Bucharest 050095, Romania

[2] Vasile Goldis Western Univ Arad, Inst Life Sci, Dept Expt & Appl Biol, 86 Rebreanu, Arad 310414, Romania

[3] Univ Reims Champagne Ardenne URCA, Inst Chim Mol Reims, CNRS UMR 7312, F-51685 Reims 2, France

来源：

POLYMERS | 2024年 / 16卷 / 08期

关键词：

gadolinium-based contrast agents; nanohydrogels; magnetic resonance imaging; CHLORIDE TOXICITY; MRI; NANOPARTICLES; EFFICIENT; CHITOSAN; CALCIUM; PRODUCTS; NANOGELS; CHITIN;

D O I：

10.3390/polym16081064

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

The aim of this study was the investigation of biochemical and histological changes induced in different tissues, as a result of the subcutaneous administration of Gd nanohydrogels (GdDOTA subset of CS-TPP/HA) in a CD-1 mouse strain. The nanohydrogels were obtained by encapsulating contrast agents (GdDOTA) in a biocompatible polymer matrix composed of chitosan (CS) and hyaluronic acid (HA) through the ionic gelation process. The effects of Gd nanohydrogels on the redox status were evaluated by measuring specific activities of the antioxidant enzymes catalase (CAT), glutathione peroxidase (GPx), and superoxide dismutase (SOD), as well as oxidative stress markers, such as reduced glutathione (GSH), malondialdehyde (MDA), advanced oxidation protein products (AOPP), and protein-reactive carbonyl groups (PRCG), in the liver, kidney, and heart tissues. The nitrosylated proteins expression were analyzed with Western Blot and the serum biochemical markers were measured with spectrophotometric methods. Also, a histological analysis of CD-1 mouse tissues was investigated. These results indicated that Gd nanohydrogels could potentially be an alternative to current MRI contrast agents thanks to their low toxicity in vivo.

引用

页数：16

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