Localization data of the T-DNA insertion site in Arabidopsis line SALK_146824C

被引：0

作者：

Belykh, Elena ^{[1
]}

Yadrikhinskiy, Kirill ^{[2
]}

Golubev, Maksim ^{[2
]}

Belykh, Nikolay ^{[2
]}

Velegzhaninov, Ilya ^{[1
]}

Garmash, Elena ^{[1
]}

机构：

[1] RAS, Komi Sci Ctr, Inst Biol, Ural Div, Kommunisticheskaya 28, Syktyvkar 167982, Russia

[2] Pitirim Sorokin Syktyvkar State Univ, Oktyabrsky Prosp 55, Syktyvkar 167001, Russia

来源：

DATA IN BRIEF | 2024年 / 54卷

基金：

俄罗斯科学基金会;

关键词：

Arabidopsis thaliana; T-DNA; Mutant line; VTC2; MUTAGENESIS; GENES;

D O I：

10.1016/j.dib.2024.110309

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The present work reports on the determination of localization of T -DNA insertion in Arabidopsis thaliana SALK_146824C line. The line is characterized as deficient in GDP -l -galactose phosphorylase 1 ( VTC2 ) gene encodes a protein involved in ascorbate biosynthesis. Primer pairs allow to distinguishing SALK_146824C from wild type plants and to the exact localization of the insertion were designed used. Sanger sequencing confirmed the location of the T -DNA insertion in the sixth exon of the gene. RT-PCR data shows nearly undetectable levels of VTC2 mRNA expression level in SALK_146824C line as compared with wild type plants of Columbia -0 line. Localization of the primer pair upstream and downstream the T -DNA insertion did not affect the expression values. (c) 2024 The Authors. Published by Elsevier Inc.

引用

页数：6

共 16 条

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